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Transcriptome profiling reveal key hub genes in co-expression networks involved in Iridoid glycosides biosynthetic machinery in Picrorhiza kurroa
Genomics ( IF 4.4 ) Pub Date : 2021-07-28 , DOI: 10.1016/j.ygeno.2021.07.024
Ashish Sharma 1 , Dipto Bhattacharyya 1 , Shilpa Sharma 1 , Rajinder Singh Chauhan 1
Affiliation  

Picrorhiza kurroa is a medicinal herb rich in hepatoprotective iridoid glycosides, picroside-I (P-I) and picroside-II (P-II). The biosynthetic machinery of picrosides is poorly understood, therefore, ‘no-direction’ gene co-expression networks were used to extract linked/closed and separated interactions in terpenoid glycosides-specific sub-networks. Transcriptomes generated from different organs, varying for P-I and P-II contents such as shoots grown at 15 and 25 °C and nursery-grown shoots, stolons, and roots resulted in 47,726, 44,958, 40,117, 66,979, and 55,578 annotated transcripts, respectively. Occurrence of 2810 ± 136 nodes and 15,626 ± 696 edges in these networks indicated intense, co-expressed, closed loop interactions. Either deregulation/inhibition of abscisic acid (ABA) biosynthesis/signaling or constitutive degradation of ABA resulted in organ-specific accumulation of P-I and P-II. Biosynthesis, condensation and glucosylation of isoprene units may occur in shoots, roots or stolons; but addition of phenylpropanoid moiety and further modification/s of the iridoid backbone occurs mainly inside vacuoles in roots.



中文翻译:

转录组分析揭示了参与环烯醚萜苷生物合成机制的共表达网络中的关键枢纽基因。

Picrorhiza kurroa是一种富含保肝环烯醚萜苷、picroside-I (PI) 和picroside-II (P-II) 的药草。苦苷的生物合成机制知之甚少,因此,“无方向”基因共表达网络被用于提取萜类苷特异性子网络中的连接/闭合和分离相互作用。由不同器官产生的转录组,PI 和 P-II 含量不同,例如在 15 和 25°C 生长的芽和苗圃生长的芽、匍匐茎和根,分别产生 47,726、44,958、40,117、66,979 和 55,578 个带注释的转录本. 在这些网络中出现 2810 ± 136 个节点和 15,626 ± 696 个边表明强烈的、共同表达的、闭环相互作用。脱落酸的放松管制/抑制(ABA) ABA 的生物合成/信号传导或组成性降解导致 PI 和 P-II 的器官特异性积累。异戊二烯单元的生物合成、缩合和糖基化可能发生在枝条、根或匍匐茎中;但是苯丙烷部分的添加和环烯醚萜骨架的进一步修饰主要发生在根部的液泡内。

更新日期:2021-08-09
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