Silica nanoparticles (SiNPs) and cadmium chloride (CdCl₂) are two important environmental pollutants. In previous research, found that SiNPs in zebrafish larvae can amplify the cardiovascular damage caused by cadmium. Whether SiNPs in the ovaries can amplify the adverse effects of cadmium on the zebrafish ovaries is worth studying problem. In this study, sexually mature female zebrafish were used as model organisms and exposed to 1 μmol/L CdCl₂ and/or 25 μg/mL SiNPs for 30 days. The results showed that the structure and function of ovaries in the sole and combined exposure groups changed significantly, resulting in reduced ovarian quality, decreased number of mature oocytes, and the development of malformed offspring. A deep-sequencing analysis showed that organisms’ lipid metabolism and transportation, estrogen metabolism, and response to the maturation, meiosis, and vitellogenin synthesis of oocytes were significantly affected by single exposure or combined exposure. These findings provide further insights into the harm of cooperation of CdCl2 and/or SiNPs to the aquatic ecosystems.

二氧化硅纳米粒子 (SiNPs) 和氯化镉 (CdCl ₂ ) 是两种重要的环境污染物。在之前的研究中，发现斑马鱼幼体中的 SiNPs 可以放大镉引起的心血管损伤。卵巢中的SiNPs是否能放大镉对斑马鱼卵巢的不利影响是值得研究的问题。在本研究中，性成熟的雌性斑马鱼被用作模式生物并暴露于 1 μmol/L CdCl ₂和/或 25 μg/mL SiNP，持续 30 天。结果表明，单独和联合暴露组卵巢结构和功能发生显着变化，导致卵巢质量下降，成熟卵母细胞数量减少，后代发育畸形。深度测序分析表明，单次暴露或联合暴露显着影响生物体的脂质代谢和运输、雌激素代谢以及对卵母细胞成熟、减数分裂和卵黄蛋白原合成的反应。这些发现为 CdCl2 和/或 SiNPs 合作对水生生态系统的危害提供了进一步的见解。