Aim

We aimed to explore the expression of miRNAs and their potential roles in the DNA damage response (DDR) induced by Cr (VI) exposure in human B lymphoblast cells (HMy2.CIR cells) and in a population of Cr (VI)-exposed humans. Methods: Differentially expressed miRNAs were found by a combination of miRNA sequencing and RT-qPCR validation in HMy2.CIR cells treated with K₂Cr₂O₇. Differentially expressed miRNAs related to DDR were selected for functional study. The expression levels of differential miRNAs were also investigated in chromate workers. Results: A total of 214 differentially expressed miRNAs were identified by sequencing, and the expression of 5 miRNAs among 25 associated with DDR was found to be consistent between sequencing and validation studies. Functional studies showed that miR-148a-3p, miR-21-5p, and miR-424-3p might be related to Cr (VI)-induced cell apoptosis, and miR-221-3p might participate in Cr (VI)-induced DDR. We also found that the expression of miR-21-5p and miR-424-3p was upregulated in chromate workers. Conclusions: Cr (VI) exposure could significantly impact miRNAs expression in vitro and in chromate workers. Functional studies showed that miR-148a-3p, miR-21-5p and miR-221-3p might take a crucial role in the cellular DDR induced by Cr (VI) exposure.

中文翻译：

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与六价铬暴露诱导的 DNA 损伤反应相关的 miRNA 的全基因组分析（DDR 相关 miRNA 响应 Cr (VI) 暴露）

目的

我们旨在探索 miRNA 的表达及其在人 B 淋巴母细胞（HMy2.CIR 细胞）和暴露于 Cr (VI) 的人群中 Cr (VI) 暴露诱导的 DNA 损伤反应 (DDR) 中的潜在作用. 方法：在用 K ₂ Cr ₂ O ₇处理的 HMy2.CIR 细胞中，通过 miRNA 测序和 RT-qPCR 验证的组合发现了差异表达的 miRNA 。选择与DDR相关的差异表达的miRNA进行功能研究。还在铬酸盐工人中研究了差异 miRNA 的表达水平。结果：通过测序共鉴定出214个差异表达的miRNA，在测序和验证研究之间发现25个与DDR相关的miRNA中5个miRNA的表达是一致的。 功能研究表明，miR-148a-3p、miR-21-5p和miR-424-3p可能与Cr(VI)诱导的细胞凋亡有关，miR-221-3p可能参与Cr(VI)诱导的细胞凋亡。复员协议。我们还发现 miR-21-5p 和 miR-424-3p 在铬酸盐工人中的表达上调。结论：Cr (VI) 暴露可显着影响体外和铬酸盐工人中miRNA 的表达。功能研究表明，miR-148a-3p、miR-21-5p 和 miR-221-3p 可能在 Cr (VI) 暴露诱导的细胞 DDR 中起关键作用。