Halal products are growing in consumer markets worldwide, and pork meat is classified as non-halal. Manufacturers of processed foods and products must ensure that their products follow Islamic dietary law because pork is prohibited for Muslims. Droplet digital polymerase chain reaction (PCR) (ddPCR) is a novel method for identifying pig species and quantifying pork products. This experiment aimed to investigate pork species and establish the proportion of pork in meat products using the mitochondrial cytochrome b gene (CYTB). The study found that the correlation coefficient between the meat weight and DNA concentration of pork was 0.997, and the correlation coefficient between the DNA concentration and the target DNA copy number of pork was 0.998. The accuracy of the ddPCR assay was verified using a sample of a known proportion of pork, and it was revealed that this method is highly precise in quantifying pork products. Nine products contained an undeclared meat proportion (90%). The limit of detection for pork was 0.0001 ng. The analysis indicated that the ddPCR assay has high accuracy and sensitivity for quantifying pork products. Therefore, the predictive model can be used in routine laboratories for quality assurance of halal food products.

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用于识别和量化猪肉产品的液滴数字聚合酶链反应分析

清真产品在全球消费市场中不断增长，猪肉被归类为非清真食品。加工食品和产品的制造商必须确保他们的产品符合伊斯兰饮食法，因为穆斯林禁止食用猪肉。液滴数字聚合酶链反应 (PCR) (ddPCR) 是一种识别猪种和量化猪肉产品的新方法。本实验旨在利用线粒体细胞色素b基因（ CYTB）调查猪肉品种并确定猪肉在肉制品中的比例). 研究发现，猪肉的肉重与DNA浓度的相关系数为0.997，猪肉DNA浓度与目标DNA拷贝数的相关系数为0.998。使用已知比例的猪肉样本验证了 ddPCR 检测的准确性，结果表明该方法在量化猪肉产品方面具有高度精确性。九种产品含有未申报的肉类比例 (90%)。猪肉的检测限为 0.0001 ng。分析表明，ddPCR 检测对猪肉产品的定量具有很高的准确性和灵敏度。因此，该预测模型可用于常规实验室，以保证清真食品的质量。