Cellulase refers to a group of enzymes like endoglucanases (CMCase), exoglucanases and β-glucosidase that have great industrial importance for the production of second-generation ethanol, e.g. The present study aimed to evaluate the CMCase and β-glucosidase activities of the fungal strain Penicillium sp. FSDE15 using different agroindustrial wastes (wheat bran, sugarcane bagasse and sugarcane straw) as carbon sources to the basal medium during Submerged Fermentation (SFm). When the medium presented wheat bran, highest CMCase and β-glucosidase yields were 1.03 U/mL (96 h) and 1.68 U/mL (168 h), respectively. For sugarcane bagasse medium, maximum enzymatic activity values were observed in 120 h culture (1.38 U/mL for CMCase and 0.55 U/mL for β-glucosidase). Sugarcane straw medium presented highest enzymatic yields after 120 h culture (1.02 U/mL of CMCase and 0.39 U/mL of β-glucosidase). The response surface methodology showed highest CMCase (1.3 U/mL) and β-glucosidase (6.5 U/mL) activities in the presence of lower levels of wheat bran (1%) (w/w), inoculum concentration of 10⁵ spores/mL, temperatures of 41 °C and 33 °C, respectively. Penicillium sp. FSDE15 presented two isoforms of CMCase (150 kDa and around 30 kDa) and one isoform of β-glucosidase (70 kDa). Our study demonstrated that the production of cellulases with high activity by Penicillium sp. FSE15 in a culture medium with agroindustrial wastes, such as wheat bran, might be a promising alternative to reduce the cost of these molecules and facilitate their use in various industrial processes.

纤维素酶是指一组酶，如内切葡聚糖酶 (CMCase)、外切葡聚糖酶和 β-葡糖苷酶，它们对第二代乙醇的生产具有重要的工业意义，例如本研究旨在评估真菌菌株的 CMCase 和 β-葡糖苷酶活性青霉菌sp. FSDE15 使用不同的农业工业废物（麦麸、甘蔗渣和甘蔗秸秆）作为水下发酵 (SFm) 期间基础培养基的碳源。当培养基呈现麦麸时，最高的 CMCase 和 β-葡萄糖苷酶产量分别为 1.03 U/mL（96 小时）和 1.68 U/mL（168 小时）。对于甘蔗渣培养基，在 120 小时培养中观察到最大酶活性值（CMCase 为 1.38 U/mL，β-葡萄糖苷酶为 0.55 U/mL）。甘蔗秸秆培养基在培养 120 小时后的酶产量最高（CMCase 为 1.02 U/mL，β-葡萄糖苷酶为 0.39 U/mL）。响应面方法显示，在较低水平的麦麸 (1%) (w/w)、接种浓度为 10 ⁵孢子数/mL，温度分别为 41 °C 和 33 °C。青霉属 FSDE15 呈现两种 CMCase 同种型（150 kDa 和约 30 kDa）和一种 β-葡萄糖苷酶同种型（70 kDa）。我们的研究表明，青霉属生产具有高活性的纤维素酶。FSE15 在含有农工业废物（如麦麸）的培养基中，可能是降低这些分子成本并促进它们在各种工业过程中使用的有前途的替代方案。