Recently there has been upsurge in reports that illicit seizures of cocaine and heroin have been adulterated with fentanyl. Surface-enhanced Raman spectroscopy (SERS) provides a useful alternative to current screening procedures that permits detection of trace levels of fentanyl in mixtures. Samples are solubilized and allowed to interact with aggregated colloidal nanostars to produce a rapid and sensitive assay. In this study, we present the quantitative determination of fentanyl in heroin and cocaine using SERS, using a point-and-shoot handheld Raman system. Our protocol is optimized to detect pure fentanyl down to 0.20 ± 0.06 ng/mL and can also distinguish pure cocaine and heroin at ng/mL levels. Multiplex analysis of mixtures is enabled by combining SERS detection with principal component analysis and super partial least squares regression discriminate analysis (SPLS-DA), which allow for the determination of fentanyl as low as 0.05% in simulated seized heroin and 0.10% in simulated seized cocaine samples.

最近，关于非法缉获的可卡因和海洛因掺有芬太尼的报道激增。表面增强拉曼光谱 (SERS) 为当前筛选程序提供了一种有用的替代方法，可以检测混合物中痕量水平的芬太尼。样品被溶解并允许与聚集的胶体纳米星相互作用以产生快速和灵敏的测定。在本研究中，我们介绍了使用 SERS 和手持式拉曼系统对海洛因和可卡因中的芬太尼进行定量测定。我们的协议经过优化，可以检测低至 0.20 ± 0.06 ng/mL 的纯芬太尼，还可以区分 ng/mL 水平的纯可卡因和海洛因。