Abstract

The halotolerant phosphate-solubilizing fungus Penicillium sp. NAUSF2 was isolated from rhizospheric soil in coastal regions of South Gujarat, India, and identified on the basis of morphological characteristics and internal transcribed spacer gene sequence (ITS1 and ITS4). The isolate solubilized rock phosphate up to 843 µM. There was drop in pH with the secretion of gluconate (31.7 mM) and oxalate (3.2 mM) mediated by the high glucose oxidase and oxaloacetate acetylhydrolase activities, respectively. This fungus also demonstrated plant growth-promoting activities such as indole-3-acetic acid (127.82 g/L) and siderophore production. Inoculation of Penicillium sp. NAUSF2 to Vigna radiata (mung bean) significantly increased plant P uptake and growth parameters. It showed reduced disease severity index due to increased levels of antioxidative enzymes and jasmonic acid when treated with the leaf spot pathogen Xanthomonas axonopodis pv. V. radiata. These results suggest the potential of Penicillium sp. NAUSF2 as a biotechnological tool to help plants to cope up with stress in saline soils and broaden the spectrum of phosphate solubilizers available for field application.

中文翻译：

---

青霉属植物的促生长活性。NAUSF2 改善缺磷盐渍土壤中的豇豆盐度胁迫

摘要

耐盐磷酸盐增溶真菌Penicillium sp。NAUSF2是从印度南古吉拉特邦沿海地区的根际土壤中分离出来的，并根据形态特征和内部转录间隔基因序列（ITS1和ITS4）进行鉴定。分离物溶解磷酸盐岩高达 843 µM。pH值随着葡萄糖酸（31.7 mM）和草酸（3.2 mM）的分泌而下降，分别由高葡萄糖氧化酶和草酰乙酸乙酰水解酶活性介导。这种真菌还表现出促进植物生长的活性，例如吲哚-3-乙酸 (127.82 g/L) 和铁载体生产。青霉菌的接种。NAUSF2 到Vigna radiata（绿豆）显着增加植物磷吸收和生长参数。当用叶斑病病原体Xanthomonas axonopodis pv处理时，由于抗氧化酶和茉莉酸的水平增加，它显示出疾病严重程度指数降低。五、辐射。这些结果表明青霉菌的潜力。NAUSF2 作为一种生物技术工具，可帮助植物应对盐渍土中的压力，并拓宽可用于田间应用的磷酸盐增溶剂的范围。