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Overexpression of tyrosine decarboxylase (MdTYDC) enhances drought tolerance in Malus domestica
Scientia Horticulturae ( IF 4.3 ) Pub Date : 2021-07-28 , DOI: 10.1016/j.scienta.2021.110425
Tengteng Gao 1 , Yanpeng Wang 1 , Yuan Liu 1 , Mengnan Ma 1 , Xianpeng Li 1 , Danni Zhang 1 , Ke Ding 1 , Chao Li 1 , Yangjun Zou 1 , Fengwang Ma 1
Affiliation  

Dopamine is produced in plants and plays a vital role in improving plant responses to abiotic stress. Tyrosine decarboxylase (TYDC) synthesizes tyramine from tyrosine, thereby providing the substrate for dopamine production. However, the function of TYDC in response to drought stress in apple plants remains unclear. In this study, transgenic apple plants that overexpressed MdTYDC were exposed to drought, in order to explore the biological function of TYDC in drought stress. Overexpression of MdTYDC significantly increased apple dopamine content. Transgenic apple plants that overexpressed MdTYDC exhibited less electrolyte leakage, a lower water loss rate, higher photosynthetic pigment contents, and enhanced photosynthetic performance under drought conditions. MdTYDC overexpression also improved reducing power by increasing the ratios of ascorbate to dehydroascorbate and reduced to oxidized glutathione. It influenced leaf water retention capacity and stomatal movement by modulating cellular abscisic acid (ABA) levels and ABA sensitivity. Transcriptomic analyses indicated that MdTYDC overexpression enhanced drought tolerance by regulating the expression of ABA- and drought-related transcription factors, including those from the NAC, ERF, and WRKY families. These results provide evidence for the involvement of MdTYDC and dopamine in enhancing the drought tolerance of apple plants.



中文翻译:

酪氨酸脱羧酶 (MdTYDC) 的过度表达增强了苹果的耐旱性

多巴胺在植物中产生,在改善植物对非生物胁迫的反应方面起着至关重要的作用。酪氨酸脱羧酶 (TYDC) 从酪氨酸合成酪胺,从而为多巴胺的产生提供底物。然而,TYDC 响应苹果植物干旱胁迫的功能仍不清楚。本研究将过表达MdTYDC 的转基因苹果植株暴露于干旱环境中,以探索 TYDC 在干旱胁迫中的生物学功能。MdTYDC 的过表达显着增加了苹果的多巴胺含量。过表达MdTYDC 的转基因苹果植株表现出较少的电解质泄漏、较低的失水率、较高的光合色素含量以及在干旱条件下增强的光合性能。MdTYDC过表达还通过增加抗坏血酸与脱氢抗坏血酸和还原为氧化型谷胱甘肽的比率来提高还原能力。它通过调节细胞脱落酸 (ABA) 水平和 ABA 敏感性来影响叶片保水能力和气孔运动。转录组学分析表明,MdTYDC过表达通过调节 ABA 和干旱相关转录因子(包括来自 NAC、ERF 和 WRKY 家族的转录因子)的表达来增强耐旱性。这些结果为MdTYDC和多巴胺参与提高苹果植物的耐旱性提供了证据。

更新日期:2021-07-28
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