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Morphometric and molecular identification of the female castes of Bombus ignitus and B. ardens (Apidae: Hymenoptera)
Journal of Asia-Pacific Entomology ( IF 1.5 ) Pub Date : 2021-07-28 , DOI: 10.1016/j.aspen.2021.07.020
Saeed Mohamadzade Namin 1, 2 , Heung-Sik Lee 3 , Minwoong Son 4 , Chuleui Jung 1, 4
Affiliation  

Among Korean bumblebees, Bombus ignitus and B. ardens are relatively abundant and important for pollination of wildflowers and agricultural crops. Although the males are easily distinguishable phenotypically, the female castes are difficult to identify from each other. Here we evaluated the value of some morphometric characters in species identification. Also, we developed a polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) to discriminate these similar species. In spite of statistically significant differences of some morphological characters between two species, overlapping quantitative traits hindered accurate identification of the species. However, using 435 bp of COI gene and AluI, BspHI and Earl restriction enzymes allowed molecular identifications of these two species with unique profiles from the digestion by these restriction enzymes. This method can also be applied for older specimens with some morphological characters damaged. We also developed species-specific primers for fast and cost-effective identification of these species.



中文翻译:

Bombus ignitus 和 B. ardens(蜂科:膜翅目)雌性种姓的形态计量学和分子鉴定

在韩国大黄蜂中,Bombus ignitusB. ardens相对丰富,对野花和农作物的授粉很重要。虽然男性在表型上很容易区分,但女性种姓很难相互识别。在这里,我们评估了一些形态特征在物种识别中的价值。此外,我们开发了聚合酶链反应,然后是限制性片段长度多态性 (PCR-RFLP),以区分这些相似的物种。尽管两个物种之间的一些形态特征在统计上存在显着差异,但重叠的数量性状阻碍了物种的准确识别。然而,使用 435 bp 的 COI 基因和 AluI、BspHI 和 Earl 限制性内切酶允许通过这些限制性内切酶消化的独特特征对这两个物种进行分子鉴定。这种方法也适用于一些形态特征受损的旧标本。我们还开发了物种特异性引物,用于快速、经济地识别这些物种。

更新日期:2021-08-26
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