The Dazl (deleted in azoospermia-like) gene encodes an RNA-binding protein containing an RNA recognition motif (RRM) and a DAZ motif. Dazl is essential for gametogenesis in vertebrates. In this study, we report the cloning of Dazl cDNA from Cynops cyanurus. Ccdazl mRNA showed a germline-specific expression pattern as expected. Ccdazl expression gradually decreased during oogenesis, suggesting that it may be involved in oocyte development. Phylogenetic analysis revealed that the Ccdazl protein shares conserved motifs/domains with Dazl proteins from other species. Cloning of Ccdazl provides a new tool to carry out comparative studies of germ cell development in amphibians.

Dazl （在无精子症样中缺失）基因编码含有 RNA 识别基序 (RRM) 和 DAZ 基序的 RNA 结合蛋白。Dazl 对脊椎动物的配子发生至关重要。在这项研究中，我们报告了从Cynops cyanurus中克隆Dazl cDNA 。如预期的那样， Ccdazl mRNA 显示出种系特异性表达模式。Ccdazl表达在卵子发生过程中逐渐下降，表明它可能参与了卵母细胞的发育。系统发育分析表明，Ccdazl 蛋白与来自其他物种的 Dazl 蛋白共享保守的基序/结构域。Ccdazl的克隆为两栖动物生殖细胞发育的比较研究提供了一种新工具。