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Fasting increases 18:2-containing phosphatidylcholines to complement the decrease in 22:6-containing phosphatidylcholines in mouse skeletal muscle.
PLOS ONE ( IF 3.7 ) Pub Date : 2021-07-26 , DOI: 10.1371/journal.pone.0255178
Nanami Senoo 1 , Takumi Akahori 1 , Hiyori Ichida 1 , Noriyuki Miyoshi 2 , Akihito Morita 1 , Takao Shimizu 3 , Hideo Shindou 3, 4 , Shinji Miura 1
Affiliation  

Fasting stimulates catabolic reactions in skeletal muscle to survive nutrient deprivation. Cellular phospholipids have large structural diversity due to various polar-heads and acyl-chains that affect many cellular functions. Skeletal muscle phospholipid profiles have been suggested to be associated with muscle adaptations to nutritional and environmental status. However, the effect of fasting on skeletal muscle phospholipid profiles remains unknown. Here, we analyzed phospholipids using liquid chromatography mass spectrometry. We determined that fasting resulted in a decrease in 22:6-containing phosphatidylcholines (PCs) (22:6-PCs) and an increase in 18:2-containing PCs (18:2-PCs). The fasting-induced increase in 18:2-PCs was sufficient to complement 22:6-PCs loss, resulting in the maintenance of the total amount of polyunsaturated fatty acid (PUFA)-containing PCs. Similar phospholipid alterations occurred in insulin-deficient mice, which indicate that these observed phospholipid perturbations were characteristic of catabolic skeletal muscle. In lysophosphatidic acid acyltransferase 3-knockout muscles that mostly lack 22:6-PCs, other PUFA-containing PCs, mainly 18:2-PCs, accumulated. This suggests a compensatory mechanism for skeletal muscles to maintain PUFA-containing PCs.

中文翻译:

禁食会增加含 18:2 的磷脂酰胆碱,以补充小鼠骨骼肌中含 22:6 的磷脂酰胆碱的减少。

禁食会刺激骨骼肌中的分解代谢反应,从而在营养缺乏的情况下存活下来。由于影响许多细胞功能的各种极性头和酰基链,细胞磷脂具有很大的结构多样性。骨骼肌磷脂谱已被认为与肌肉对营养和环境状况的适应有关。然而,禁食对骨骼肌磷脂谱的影响仍然未知。在这里,我们使用液相色谱质谱法分析了磷脂。我们确定禁食导致含 22:6 的磷脂酰胆碱 (PC) (22:6-PC) 减少,而含 18:2 的 PC (18:2-PC) 增加。禁食引起的 18:2-PCs 增加足以弥补 22:6-PCs 的损失,导致含有多不饱和脂肪酸 (PUFA) 的 PC 总量保持不变。在胰岛素缺乏的小鼠中发生了类似的磷脂改变,这表明这些观察到的磷脂扰动是分解代谢骨骼肌的特征。在大多数缺乏 22:6-PCs 的溶血磷脂酸酰基转移酶 3-敲除肌肉中,其他含有 PUFA 的 PCs,主要是 18:2-PCs,积累。这表明骨骼肌维持含 PUFA 的 PC 的补偿机制。积累。这表明骨骼肌维持含 PUFA 的 PC 的补偿机制。积累。这表明骨骼肌维持含 PUFA 的 PC 的补偿机制。
更新日期:2021-07-26
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