The key challenge in tissue engineering is to develop structures with sufficient characteristics that will replicate the natural extracellular matrix (ECM) to promote tissue regeneration. Copper and manganese minerals substituted hydroxyapatite (CuMn-HA)/chitosan (CTS)–polyvinyl pyrrolidone (PVD) were fabricated by sol–gel and solvent casting techniques, respectively. Fabricated biocomposites have been identified by the use of Fourier transform infrared (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) for the analysis of functional groups, crystalline phases and surface morphology. The XRD and FTIR spectra of the biocomposite revealed the existence of functional groups and crystalline phases in the biocomposites. The SEM images revealed biocomposite and inclusion of CuMn-HA in rough morphology. Physicochemical characteristics such as swelling and compressive strength have been examined in the preparation of biocomposite materials. Biocomposites (10, 20, 30 wt% of CuMn-HA biocomposite) exhibited great material characteristics where 30% displayed the minimum swelling and a compressive property of 71 MPa was tested. It displayed the highest inhibition zone towards bacteria population and was also found to be blood-compatible with a haemolytic value of only about 2.5% relative to other compounds. Finally, in-vitro cell cultures were performed in biocomposite scaffolds to determine the effects of CuMn-HA on the proliferation of osteoblast cells, resulting in biocomposites contrast to pristine PVD/CTS.

组织工程的关键挑战是开发具有足够特性的结构，以复制天然细胞外基质 (ECM) 以促进组织再生。铜和锰矿物取代的羟基磷灰石（CuMn-HA）/壳聚糖（CTS）-聚乙烯吡咯烷酮（PVD）分别通过溶胶-凝胶和溶剂浇铸技术制备。已通过使用傅里叶变换红外 (FTIR)、X 射线衍射 (XRD) 和扫描电子显微镜 (SEM) 来分析官能团、结晶相和表面形态来鉴定制造的生物复合材料。生物复合材料的 XRD 和 FTIR 光谱表明生物复合材料中存在官能团和晶相。SEM 图像显示了生物复合材料和 CuMn-HA 的粗略形态。在生物复合材料的制备过程中已经检查了物理化学特性，例如膨胀和压缩强度。生物复合材料（10、20、30 wt% 的 CuMn-HA 生物复合材料）表现出很好的材料特性，其中 30% 显示出最小的膨胀和 71 MPa 的压缩性能。它显示出对细菌种群的最高抑制区，并且还发现与血液相容，相对于其他化合物，溶血值仅为约 2.5%。最后，它显示出对细菌种群的最高抑制区，并且还发现与血液相容，相对于其他化合物，溶血值仅为约 2.5%。最后，它显示出对细菌种群的最高抑制区，并且还发现与血液相容，相对于其他化合物，溶血值仅为约 2.5%。最后，在生物复合支架中进行体外细胞培养，以确定 CuMn-HA 对成骨细胞增殖的影响，产生与原始 PVD/CTS 形成对比的生物复合材料。