This study focused on the effect of Noni, Morinda citrifolia fruit extract supplementation on the growth and the hepatopancreas function of Whiteleg shrimp, Penaeus vannamei Post Larvae (PL). The crude extract of M. citrifolia fruit was obtained through drying and methanol extraction followed by concentration through rotary evaporation. The extracts were incorporated into commercial shrimp pellet at the concentrations of 0%, 1%, 2%, 3%, 4% and 5%. Feeding trial was done for 30 days and shrimp samples were collected at 5 days interval for growth performance determination and physiology analysis on the hepatopancreas. Despite the inconsistency in growth performances during the early feeding period, the extract supplementation did improved the growth of P. vannamei PL. Significant differences in the digestive enzymes and antioxidant enzymes activities between treatments were observed. The correlation results showed only amylase were correlated with M. citrifolia fruit extract supplementation. In terms of the relationship with growth, amylase and lipase showed correlation with the BW changes of P. vannamei PL while trypsin otherwise. Antioxidant enzymes were strongly correlated with the concentration of M. citrifolia fruit extract supplemented and growth of P. vannamei PL. This indicates that the growth of shrimp was improved with increased antioxidant enzyme activities. The number of hepatopancreatic cells (B, R & E cells) also increased significantly with the increasing concentration of M. citrifolia supplementation during the 30-day feeding trial. Similarly, strong correlation was observed between the hepatopancreatic cells and the growth of P. vannamei PL. The appearance of cell atrophies and haemocytic infiltration in the hepatopancreas might indicate the stimulation of innate defense mechanism in the hepatopancreas by the extract supplementation.

本研究的重点是添加诺丽、巴戟天果实提取物对南美白对虾后期幼虫 (PL)的生长和肝胰腺功能的影响。柑桔果实粗提物经干燥、甲醇提取、旋转蒸发浓缩得到。提取物以 0%、1%、2%、3%、4% 和 5% 的浓度掺入商业虾丸中。饲养试验进行了 30 天，每隔 5 天收集虾样品用于肝胰腺的生长性能测定和生理学分析。尽管在早期饲养期间生长性能不一致，但提取物的补充确实改善了南美白对虾PL。观察到处理之间消化酶和抗氧化酶活性的显着差异。相关性结果表明，只有淀粉酶与M. citrifolia果实提取物的补充相关。在与生长的关系方面，淀粉酶和脂肪酶与南美白对虾的体重变化呈相关性，而胰蛋白酶则与此相关。抗氧化酶与添加柑橘叶海棠果实提取物的浓度和南美白对虾的生长密切相关PL。这表明虾的生长随着抗氧化酶活性的增加而得到改善。在 30 天喂养试验期间，肝胰腺细胞（B、R 和 E 细胞）的数量也随着M. citrifolia补充剂浓度的增加而显着增加。同样，在肝胰腺细胞和南美白对虾PL的生长之间也观察到了很强的相关性。肝胰腺中细胞萎缩和血细胞浸润的出现可能表明补充提取物刺激了肝胰腺中的先天防御机制。