Pantothenic acid is an essential metabolite found throughout all branches of life. Although the enzymes responsible for pantothenic biosynthesis have been characterized, those leading to its biodegradation remain poorly understood. In the study described herein, we showed that use of a “genomic enzymology” strategy enabled identification of four biodegradation pathway genes, which were then confirmed by using kinetic analysis of the purified recombinant enzymes encoded in Ochrobactrum anthropi. The reconstituted pathway converts pantothenic acid to β-alanine and (R)-pantoate, and then (R)-pantoate to aldopentoate, which is transformed to (R)-3,3-dimethylmalate and hence to α-ketoisovalerate. The pathway genes are common to Proteobacterial genomes in which they are not colocated.

中文翻译：

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Ochrobactrum anthropi 中泛酸降解途径的体外重建

泛酸是一种在生命的所有分支中都能找到的必需代谢物。尽管负责泛酸生物合成的酶已被表征，但导致其生物降解的酶仍然知之甚少。在本文所述的研究中，我们表明使用“基因组酶学”策略能够鉴定四种生物降解途径基因，然后通过使用在人类 Ochrobactrum anthropi 中编码的纯化重组酶的动力学分析进行确认。重构途径将泛酸转化为 β-丙氨酸和 ( R ) -泛解酸，然后 ( R ) -泛解酸转化为戊醛酸，后者转化为 ( R)-3,3-二甲基苹果酸酯，从而生成α-酮异戊酸酯。通路基因对于它们不共存的变形菌基因组是常见的。