An ultrasensitive electrochemical biosensor for HPV16 oncogene was explored. Hairpin DNA-1, which can specifically bind with HPV16 oncogene, was fixed on the surface of gold electrode. Two hairpin DNAs underwent catalytic hairpin assembling with hairpin DNA-1 to construct Y-shaped DNA nanostructure, liberating HPV16 oncogene for target recycling. The 3’ terminus of Y-shaped DNA nanostructure was prolonged under the catalysis of terminal deoxynucleotidyl transferase. Methylene blue was adsorbed onto DNA nanostructure to generate characteristic differential pulse voltammetry signal. This signal was increased with the concentration of HPV16 oncogene, and the detection limit of HPV16 oncogene was as low as 0.19 fM.

中文翻译：

---

基于Y型DNA催化发夹组装和无模板DNA延伸反应的HPV16癌基因超灵敏电化学生物传感器

探索了一种用于 HPV16 癌基因的超灵敏电化学生物传感器。与HPV16癌基因特异性结合的发夹DNA-1固定在金电极表面。两个发夹 DNA 与发夹 DNA-1 进行催化发夹组装，构建 Y 形 DNA 纳米结构，释放 HPV16 癌基因用于靶标回收。Y型DNA纳米结构的3'末端在末端脱氧核苷酸转移酶的催化下被延长。亚甲基蓝被吸附到 DNA 纳米结构上以产生特征差分脉冲伏安信号。该信号随HPV16癌基因浓度的增加而增强，HPV16癌基因的检出限低至0.19 fM。