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Isolation and characterization of 38 SNP markers for the black rockfish, Sebastes schlegelii by next-generation sequencing
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2021-07-27 , DOI: 10.1007/s12686-021-01226-3
Xianggang Gao 1 , Xiangbo Bao 1 , Wei Sun 1 , Yunfeng Li 1 , Zongying Liu 1 , Weidong Liu 1
Affiliation  

The black rockfish (Sebastes schlegelii) is an economically important species. However, wild S. schlegelii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of S. schlegelii. In this study, 38 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1400 to 0.6400 and 0.1487 to 0.4978, respectively. The minor allele frequency raged from 0.1429 to 0.4694. Polymorphic information content ranged from 0.174 to 0.365. Four SNPs were found to be deviated significantly from the HWE (P < 0.05). These SNP markers will serve as a useful tool for genetic studies and population evaluation aimed at the conservation of S. schlegelii.



中文翻译:

通过下一代测序分离和表征黑石斑鱼 Sebastes schlegelii 的 38 个 SNP 标记

黑石斑鱼(Sebastes schlegelii)是一种重要的经济物种。然而,近年来,由于人类干扰和栖息地破坏,野生S. schlegelii资源急剧下降。因此,保护S. schlegelii的现有资源至关重要。在这项研究中,基于限制性位点相关 DNA 测序开发了 38 个新的单核苷酸多态性 (SNP) 标记。结果表明,观察到的杂合度和预期的杂合度分别在 0.1400 到 0.6400 和 0.1487 到 0.4978 之间。次要等位基因频率从 0.1429 到 0.4694。多态信息含量从 0.174 到 0.365 不等。发现四个 SNP 显着偏离 HWE(P < 0.05)。这些 SNP 标记将作为遗传研究和种群评估的有用工具,旨在保护S. schlegelii

更新日期:2021-07-27
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