Abstract

Green synthesis of silver nanoparticles using water extract of Bistorta officinalis (BO/Ag-NPs) and evaluation of toxicity and its pro-apoptotic effects. BO/Ag-NPs were synthesized and characterized by DLS, FTIR, XRD, EDX, TEM and FESEM. Then, toxicity and apoptotic effect of BO/Ag-NPs were assessed by MTT, qPCR, flow cytometry and AO/PI methods. Spherical BO/Ag-NPs with a size of 29 nm, PDI of 0.3 and ζ potential of −27.06 mV were synthesized. Medium concentrations of BO/Ag-NPs on MDA-mb231 and HDF cells were reported about 6.1 and >50 µg.ml respectively. Increase the rate of apoptotic cells in AO/PI staining and flow cytometry and upregulation of caspase 3,9 and Bax genes and down regulation of Bcl-2 gene confirmed the activated of intrinsic pathway in BO/Ag-NPs treated cells. Due to the biological effects of BO/Ag-NP, these nanoparticles could be a promising factor in cancer therapy.

摘要

青蒿水提液绿色合成银纳米粒子（BO / Ag-NPs）和毒性评估及其促凋亡作用。合成了 BO/Ag-NPs，并通过 DLS、FTIR、XRD、EDX、TEM 和 FESEM 对其进行了表征。然后，通过MTT、qPCR、流式细胞术和AO/PI方法评估BO/Ag-NPs的毒性和凋亡作用。合成了尺寸为 29 nm、PDI 为 0.3 和 ζ 电位为 -27.06 mV 的球形 BO/Ag-NP。据报道，MDA-mb231 和 HDF 细胞上 BO/Ag-NPs 的中等浓度分别约为 6.1 和 >50 µg.ml。在 AO/PI 染色和流式细胞术中增加凋亡细胞的速率以及 caspase 3,9 和 Bax 基因的上调和 Bcl-2 基因的下调证实了 BO/Ag-NPs 处理的细胞中内在途径的激活。由于 BO/Ag-NP 的生物学效应，这些纳米颗粒可能成为癌症治疗中的一个有希望的因素。