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Role of laminin and collagen chains in human spermatogenesis – Insights from studies in rodents and scRNA-Seq transcriptome profiling
Seminars in Cell & Developmental Biology ( IF 7.3 ) Pub Date : 2021-07-27 , DOI: 10.1016/j.semcdb.2021.07.011
Xiaolong Wu 1 , Sheng Gao 2 , Lingling Wang 1 , Tiao Bu 2 , Siwen Wu 3 , Liwei Zhou 2 , Jie Shi 2 , Di Wu 2 , Fei Sun 2 , C Yan Cheng 3
Affiliation  

Studies have demonstrated that biologically active fragments are generated from the basement membrane and the Sertoli cell-spermatid adhesion site known as apical ectoplasmic specialization (apical ES, a testis-specific actin-based anchoring junction) in the rat testis. These bioactive fragments or peptides are produced locally across the seminiferous epithelium through proteolytic cleavage of constituent proteins at the basement membrane and the apical ES. Studies have shown that they are being used to modulate and coordinate cellular functions across the seminiferous epithelium during different stages of the epithelial cycle of spermatogenesis. In this review, we briefly summarize recent findings based on studies using rat testes as a study model regarding the role of these bioactive peptides that serve as a local regulatory network to support spermatogenesis. We also used scRNA-Seq transcriptome datasets in the public domain for OA (obstructive azoospermia) and NAO (non-obstructive azoospermia) human testes versus testes from normal men for analysis in this review. It was shown that there are differential expression of different collagen chains and laminin chains in these testes, suggesting the possibility of a similar local regulatory network in the human testis to support spermatogenesis, and the possible disruption of such network in men is associated with OA and/or NOA.



中文翻译:

层粘连蛋白和胶原链在人类精子发生中的作用——啮齿动物和 scRNA-Seq 转录组分析研究的见解

研究表明,生物活性片段是由大鼠睾丸中的基底膜和称为顶端外质特化(顶端 ES,一种睾丸特异性肌动蛋白锚定连接)的支持细胞-精细胞粘附位点产生的。这些生物活性片段或肽通过在基底膜和顶端 ES 处的组成蛋白的蛋白水解切割在生精上皮局部产生。研究表明,它们被用于在精子发生上皮周期的不同阶段调节和协调生精上皮细胞的细胞功能。在本次审查中,我们简要总结了基于使用大鼠睾丸作为研究模型的研究的最新发现,这些研究模型涉及这些生物活性肽作为支持精子发生的局部调节网络的作用。我们还使用公共领域的 scRNA-Seq 转录组数据集来分析 OA(阻塞性无精子症)和 NAO(非阻塞性无精子症)人类睾丸与正常男性的睾丸,以在本次审查中进行分析。结果表明,这些睾丸中存在不同胶原蛋白链和层粘连蛋白链的差异表达,这表明人类睾丸中可能存在类似的局部调节网络来支持精子发生,并且男性这种网络的可能破坏与 OA 和/ 或 NOA。我们还使用公共领域的 scRNA-Seq 转录组数据集来分析 OA(阻塞性无精子症)和 NAO(非阻塞性无精子症)人类睾丸与正常男性的睾丸,以在本次审查中进行分析。结果表明,这些睾丸中存在不同胶原蛋白链和层粘连蛋白链的差异表达,这表明人类睾丸中可能存在类似的局部调节网络来支持精子发生,并且男性这种网络的可能破坏与 OA 和/ 或 NOA。我们还使用公共领域的 scRNA-Seq 转录组数据集来分析 OA(阻塞性无精子症)和 NAO(非阻塞性无精子症)人类睾丸与正常男性的睾丸,以在本次审查中进行分析。结果表明,这些睾丸中存在不同胶原蛋白链和层粘连蛋白链的差异表达,这表明人类睾丸中可能存在类似的局部调节网络来支持精子发生,并且男性这种网络的可能破坏与 OA 和/ 或 NOA。

更新日期:2021-07-27
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