The finding of conjoined oocytes is a rare occurrence that accounts for only 0.3% of all human retrieved oocytes. This phenomenon is quite different from that of a traditional single oocyte emanating from one follicle, and may result in dizygotic twins and mosaicism. Given the insufficient evidence on how to approach conjoined oocytes, their fate is variable among different in vitro fertilization (IVF) centres. In this observational report, we propose a new protocol for the use of these conjoined oocytes using intracytoplasmic sperm injection (ICSI), laser-cutting technique and next-generation sequencing (NGS). The first case report demonstrates that conjoined oocytes can penetrate their shared zona pellucida (ZP) at Day 6. The second case is that of a 25-year-old female patient who underwent a successful embryo transfer cycle after removal of one oocyte in which a pair of conjoined human oocytes underwent ICSI, laser-cutting separation and NGS testing. The patient achieved pregnancy and gave birth to single healthy female originally derived from conjoined oocytes. This case provided a means through which normal pregnancy may be achieved from conjoined oocytes using laser-cutting separation techniques. The protocol described may be especially beneficial to patients with a limited number of oocytes.

联合卵母细胞的发现是一种罕见的现象，仅占人类所有取回卵母细胞的 0.3%。这种现象与传统的单个卵母细胞从一个卵泡中产生的现象有很大不同，并可能导致双卵双胞胎和嵌合体。鉴于关于如何接近联合卵母细胞的证据不足，它们的命运在不同的体外受精（IVF）中心。在这份观察报告中，我们提出了一种使用胞浆内精子注射 (ICSI)、激光切割技术和下一代测序 (NGS) 来使用这些联合卵母细胞的新方案。第一个病例报告表明，在第 6 天，联合卵母细胞可以穿透其共享的透明带 (ZP)。第二个病例是一名 25 岁的女性患者，她在取出一个卵母细胞后经历了成功的胚胎移植周期，其中一对连体人类卵母细胞进行了 ICSI、激光切割分离和 NGS 测试。患者成功怀孕并生下了最初来自连体卵母细胞的单一健康女性。该案例提供了一种方法，通过该方法可以使用激光切割分离技术从联合卵母细胞中实现正常妊娠。