Background

MiRNAs belong to non-coding RNAs that are involved in cancer development. Acting as a mediator, they could regulate the expression level of numerous gens. However, the expression and function of miR-1299 in gastric cancer (GC) are not clear.

Objective

To explore the role of miR-1299 in the process of GC.

Methods

We detected the levels of miR-1299 in clinical samples of GC and investigated the role of miR-1299 in the regulation of the GC cells proliferation, apoptosis and metastasis. Luciferase reporter assay was employed to verify the target of miR-1299. Additionally, the proliferation, apoptosis and metastasis of AGS and SGC7901 cells were analyzed after the overexpression of miR-1299.

Results

Our study showed the expression of miR-1299 was decreased in GC tissues and cell lines. It indicated that the cell proliferation, migration and invasion was inhibited, while the cell apoptosis was promoted by the over-expressed miR-1299. Also, we found that miR-1299 could directly target the 3′-untranslated region (3′UTR) of ARF6 genes. In addition, rescue assay demonstrated that miR-1299 overexpression promoted the cell apoptosis and inhibited cell growth, which could be attenuated by the overexpression of ARF6.

Conclusions

These findings indicate that miR-1299 regulates cell progression in GC by targeting ARF6 genes, and suggest that miR-1299 may be a tumor suppressor in the GC progression.

中文翻译：

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MiR-1299 作为肿瘤抑制因子通过靶向 ARF6 抑制胃癌的增殖和转移

背景

miRNA属于参与癌症发展的非编码RNA。作为中介，它们可以调节众多基因的表达水平。然而，miR-1299在胃癌（GC）中的表达和功能尚不清楚。

客观的

探讨miR-1299在GC过程中的作用。

方法

我们检测了 GC 临床样本中 miR-1299 的水平，并研究了 miR-1299 在调节 GC 细胞增殖、凋亡和转移中的作用。荧光素酶报告基因检测用于验证 miR-1299 的靶标。此外，在过表达 miR-1299 后，分析了 AGS 和 SGC7901 细胞的增殖、凋亡和转移。

结果

我们的研究表明 miR-1299 在 GC 组织和细胞系中的表达降低。表明过表达的miR-1299可抑制细胞增殖、迁移和侵袭，促进细胞凋亡。此外，我们发现 miR-1299 可以直接靶向 ARF6 基因的 3'-非翻译区 (3'UTR)。此外，救援试验表明 miR-1299 过表达促进细胞凋亡并抑制细胞生长，这可以通过 ARF6 的过表达而减弱。

结论

这些发现表明 miR-1299 通过靶向 ARF6 基因调节 GC 中的细胞进展，并表明 miR-1299 可能是 GC 进展中的肿瘤抑制因子。