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Transient propagation of BmLV and dysregulation of gene expression in nontarget cells following BmLV infection
Journal of Asia-Pacific Entomology ( IF 1.5 ) Pub Date : 2021-07-27 , DOI: 10.1016/j.aspen.2021.07.017
Yongjie Feng 1 , Xing Zhang 1 , Dhiraj Kumar 1, 2 , Sulan Kuang 1 , Bo Liu 1 , Xiaolong Hu 1, 3 , Min Zhu 1 , Zi Liang 1 , Guangli Cao 1 , Renyu Xue 1 , Chengliang Gong 1, 3, 4
Affiliation  

Bombyx mori latent virus (BmLV), a novel positive-strand RNA virus was first identified in the B. mori cultured BmN cell line. Whether the infectivity of BmLV to silkworm larvae and non-silkworm cells is connected with dysregulation of gene expression are not well understood. A complete sequence of BmLV genomic RNA was identified and revealed that a fragment with 495 nt in length was deleted from the RNA-dependent RNA polymerase (RdRp) gene in some BmLV genomic RNAs. Studies on the infectivity of BmLV to nontarget cells showed that BmLV can infect silkworm larvae, Spodoptera frugiperda Sf-9 and H1299 lung cancer cells with transient propagation. The dysregulation of gene expression of Sf-9 cells followed by BmLV infection was analyzed. Out of 743 differentially expressed genes (DEGs), 300 were upregulated and 443 were downregulated. Gene Ontology (GO) analysis indicated the DEGs were enriched into oxidoreductase activity for CH-NH2 group donors, glutamate biosynthetic process, response to stress and proteasome core complex. KEGG enrichment analysis showed that DEGs were mainly enriched into sulfur metabolism, RNA degradation, proteasome, pentose and glucuronate interconversions. Undesirable nutrients and temperature factors contributed to the propagation of BmLV in Sf-9 cells. Additionally, the Imd and RNAi pathways were activated by BmLV infection without stimulating Toll and JAK-STAT pathways. Therefore, it is suggested that BmLV is originated from plants, which can enter nontarget cells with transient propagation. The transient infection of BmLV may not only be regulated by Imd and RNAi immune pathways but also mediated by dysregulation of gene expression.



中文翻译:

BmLV 感染后非靶细胞中 BmlV 的瞬时传播和基因表达失调

家蚕潜伏病毒 (BmLV) 是一种新型正链 RNA 病毒,首次在家蚕培养的 BmN 细胞系中得到鉴定。BmLV 对家蚕幼虫和非家蚕细胞的感染性是否与基因表达失调有关尚不清楚。鉴定了 BmLV 基因组 RNA 的完整序列,并揭示了从一些 BmLV 基因组 RNA 中依赖 RNA 的 RNA 聚合酶 (RdRp) 基因中删除了长度为 495 nt 的片段。BmLV对非靶细胞的感染性研究表明BmLV可感染家蚕幼虫草地夜蛾Sf-9 和 H1299 肺癌细胞瞬时增殖。分析了 BmLV 感染后 Sf-9 细胞基因表达的失调。在 743 个差异表达基因 (DEG) 中,300 个上调,443 个下调。基因本体论 (GO) 分析表明 DEG 富集了 CH-NH 2 的氧化还原酶活性组供体、谷氨酸生物合成过程、应激反应和蛋白酶体核心复合物。KEGG富集分析表明DEGs主要富集于硫代谢、RNA降解、蛋白酶体、戊糖和葡萄糖醛酸的相互转化。不良的营养和温度因素有助于 BmLV 在 Sf-9 细胞中的传播。此外,Imd 和 RNAi 通路被 BmLV 感染激活,而不刺激 Toll 和 JAK-STAT 通路。因此,建议BmLV起源于植物,它可以通过瞬时传播进入非靶细胞。BmLV 的瞬时感染可能不仅受 Imd 和 RNAi 免疫途径的调节,还可能由基因表达的失调介导。

更新日期:2021-08-26
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