Surface-enhanced Raman scattering (SERS) is a mature vibrational spectroscopic technique, and its application in chemical, material, especially life sciences is increasing rapidly. SERS alone cannot meet the demands of current analysis, while the desirable features of nucleic acid isothermal amplification (NAIA) make the simple, economical and flexible SERS become a reality. SERS has been combined with NAIA to provide researchers with more sensitive and precise analysis specifications in biological samples. However, there is no comprehensive review of the SERS analysis combined with NAIA yet. The present review introduces the development of SERS briefly and summarizes the necessary theory for understanding SERS and NAIA. The SERS analyses integrated with NAIA are the centerpiece of this review. They cover a broad range of topics, for example, hybridization chain reaction (HCR), catalytic hairpin assembly (CHA), entropy-driven catalytic reaction (EDR), strand displacement amplification (SDA), recombinase polymerase amplification (RPA), exponential amplification reaction (EXPAR), rolling circle amplification (RCA), and loop-mediated isothermal amplification (LAMP). In the end, the challenges and prospective opinions of the SERS in application are discussed.

表面增强拉曼散射（SERS）是一种成熟的振动光谱技术，其在化学、材料特别是生命科学领域的应用正在迅速增加。单靠 SERS 无法满足当前分析的需求，而核酸等温扩增 (NAIA) 的理想特性使简单、经济和灵活的 SERS 成为现实。SERS 已与 NAIA 相结合，可为研究人员提供更灵敏、更精确的生物样品分析规范。然而，目前还没有对结合 NAIA 的 SERS 分析进行全面审查。本综述简要介绍了 SERS 的发展，并总结了理解 SERS 和 NAIA 的必要理论。与 NAIA 集成的 SERS 分析是本次审查的核心。它们涵盖了广泛的主题，例如，杂交链反应 (HCR)、催化发夹组装 (CHA)、熵驱动催化反应 (EDR)、链置换扩增 (SDA)、重组酶聚合酶扩增 (RPA)、指数扩增反应 (EXPAR)、滚环扩增 (RCA)和环介导等温扩增 (LAMP)。最后讨论了SERS在应用中的挑战和展望。