Abstract

In order to further understanding of how alga are adjusted to chromium(VI) toxicity at the level of photosynthesis, the pigment content and chlorophyll a (Chla) fluorescence parameters, were studied in Macrocystis pyrifera blades treated with 0, 0.5, 2, 10 and 20 mg/L Cr (VI) solution for 24 h. Results showed that these parameters were clearly affected by different concentrations of Cr (VI The pigment contents and Chla fluorescence parameters were clearly decreased in M. pyrifera exposed to 20 mg/L Cr (VI). Furthermore, the present study provided novel insights into Cr(VI) toxicity at the proteomic level using TMT-based technology after M. pyrifera was irrigated with 0 or 20 mg/L Cr(VI) solution for 24 h. The decreases in pigment content and Chl a fluorescence parameters resulted from alternations of the related proteins involved in pigment synthesis and photosynthetic processes. Expression of 227 different proteins was detected. Analysis of significant changes in KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways showed that M. pyrifera is able to reorganize its proteome in favor of photosynthesis, carbon fixation, metabolic pathways, oxidative phosphorylation, biosynthesis of amino acids, carbon metabolism, ribosome and biosynthesis of secondary metabolites under Cr(VI) stress. These findings could provide valuable information to elucidate the mechanism of Cr(VI) toxicity in M. pyrifera.

中文翻译：

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铬 (VI) 胁迫下 Macrocystis pyrifera 的生理和蛋白质组学变化

摘要

为了进一步了解藻类如何在光合作用水平上调节六价铬毒性，研究了用0、0.5、2、10处理的Macrocystis pyrifera叶片中的色素含量和叶绿素a (Chl a ) 荧光参数和 20 mg/L Cr (VI) 溶液 24 小时。结果表明，这些参数明显受到不同浓度Cr (VI) 的影响。暴露于 20 mg/L Cr (VI) 的M. pyrifera的色素含量和 Chl a荧光参数明显降低。此外，本研究提供了新的见解M. pyrifera之后使用基于 TMT 的技术在蛋白质组水平上的 Cr(VI) 毒性用 0 或 20 毫克/升 Cr(VI) 溶液冲洗 24 小时。色素含量和 Chl a荧光参数的降低是由参与色素合成和光合作用过程的相关蛋白质的变化引起的。检测到 227 种不同蛋白质的表达。KEGG（京都基因和基因组百科全书）途径的显着变化分析表明，M. pyrifera能够重组其蛋白质组，有利于光合作用、碳固定、代谢途径、氧化磷酸化、氨基酸生物合成、碳代谢、核糖体和Cr(VI) 胁迫下次生代谢物的生物合成。这些发现可为阐明M. pyrifera中 Cr(VI) 毒性机制提供有价值的信息。