We prepared nanostructured lipid carriers (NLC) to promote skin permeation of Corylin so that it can increase its effect on photoaging. Corylin-NLCs were prepared and characterized based on morphology, particle size, zeta potentials, FTIR and DSC. In vitro, we assess the cytotoxicity and lactate dehydrogenase (LDH) of HaCaT cells irradiated by UVB. Expression of antioxidant enzymes was evaluated by commercial kits. The effects of Corylin-NLC on apoptosis were confirmed by flow cytometry and western blotting. In vivo, we use UV irradiated mouse as the oxidative stress model to assess the therapeutic effect of Corylin loaded NLC gel. We identified the Corylin-NLCs can significantly suppress the LDH release, decrease MDA content, increase in CAT, SOD, GSH-Px activity, increase the expression of Bcl-2/Bax protein and reduce the expression of cleaved caspase-3/caspase-3 protein on UVB induced HaCaT cells. The histopathological lesions were significantly improved and observably decreased MDA level, increase in antioxidant enzymes activity in serum of mice by pretreatment of Corylin-NLCs gel. Overall, this study proposes a promising strategy for improving the therapeutic efficacy of photoaging.

我们制备了纳米结构脂质载体 (NLC) 以促进 Corylin 的皮肤渗透，从而增加其对光老化的影响。基于形态、粒径、zeta 电位、FTIR 和 DSC 制备和表征 Corylin-NLC。在体外，我们评估了 UVB 照射的 HaCaT 细胞的细胞毒性和乳酸脱氢酶 (LDH)。通过商业试剂盒评估抗氧化酶的表达。通过流式细胞术和蛋白质印迹证实了 Corylin-NLC 对细胞凋亡的影响。在体内，我们使用紫外线照射的小鼠作为氧化应激模型来评估加载 Corylin 的 NLC 凝胶的治疗效果。我们发现 Corylin-NLCs 可以显着抑制 LDH 释放，降低 MDA 含量，增加 CAT、SOD、GSH-Px 活性，增加 Bcl-2/Bax 蛋白的表达，降低 UVB 诱导的 HaCaT 细胞上裂解的 caspase-3/caspase-3 蛋白的表达。Corylin-NLCs凝胶预处理显着改善了组织病理学病变，并明显降低了MDA水平，增加了小鼠血清中抗氧化酶活性。总体而言，这项研究提出了一种提高光老化治疗效果的有希望的策略。