The impact of hydrophilic polymers in an organosilica matrix on the features and performance of immobilized methylotrophic yeast cells used as biocatalysts was investigated and described. Yeast cells were immobilized in a matrix made of tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES) by one-step sol-gel route of synthesis in the presence of polyethylene glycol (PEG) or polyvinyl alcohol (PVA). Organosilica shells were spontaneously built around cells as a result of yeast immobilization at a TEOS to MTES ratio of 85/15 vol% and hydrophilic polymer (PEG or PVA). As a structure-directing agent, PVA produces organosilica films. Stable high-performance biocatalysts active for one year, if stored at −18 °C, have been obtained by entrapment of methylotrophic yeast cells. A trickling biofilter with and without active aeration was designed using entrapped yeast cells to treat methanol polluted wastewater. A biofilter model with active aeration could halve methanol input thus demonstrating better performance compared to treatment without active aeration.

研究和描述了有机二氧化硅基质中的亲水聚合物对用作生物催化剂的固定化甲基营养酵母细胞的特征和性能的影响。在聚乙二醇 (PEG) 或聚乙烯醇 (PVA) 存在下，通过一步溶胶-凝胶合成途径，将酵母细胞固定在由四乙氧基硅烷 (TEOS) 和甲基三乙氧基硅烷 (MTES) 制成的基质中。由于 TEOS 与 MTES 的比例为 85/15 vol% 和亲水聚合物（PEG 或 PVA），酵母在细胞周围自发地构建了有机硅壳。作为结构导向剂，PVA 可生产有机二氧化硅薄膜。如果在-18°C 下储存，则可通过捕获甲基营养酵母细胞获得活性一年的稳定高性能生物催化剂。设计了一种带有和不带有主动曝气的滴流生物过滤器，使用捕获的酵母细胞来处理甲醇污染的废水。具有主动曝气的生物过滤器模型可以将甲醇输入量减半，因此与没有主动曝气的处理相比，表现出更好的性能。