Aim: Metabolic reprogramming has recently attracted extensive attention for understanding cancer development. We aimed to demonstrate a genomic and transcriptomic landscape of metabolic reprogramming underlying liver cancer cell lines.
Methods: We investigated metabolic aberrant at both the transcriptome and genome levels using transcriptome and whole-exome sequencing data from 12 human liver cancer cell lines (hLCCLs) and one normal liver cell line.
Results: Three subgroups of hLCCLs characterized from transcriptome sequencing data exhibit significantly different aberrations in various metabolic processes, including amino acid, lipid, energy, and carbohydrate metabolism. Furthermore, whole-exome sequencing revealed distinct mutational signatures among different subgroups of hLCCLs and identified a total of 19 known driver genes implicated in metabolism.
Conclusion: Our findings highlighted differential metabolic mechanisms in the development of liver cancer and provided a resource for further investigating its metabolic mechanisms.

Keywords: human liver cancer cell lines (hLCCLs), transcriptome, whole-exome, sequencing, tumor metabolism


中文翻译：

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DNA 和 RNA 测序概括了肝癌细胞系中的异常肿瘤代谢

目的：代谢重编程最近引起了广泛关注，以了解癌症的发展。我们旨在展示肝癌细胞系代谢重编程的基因组和转录组学景观。
方法：我们使用来自 12 种人类肝癌细胞系 (hLCCL) 和一种正常肝细胞系的转录组和全外显子组测序数据研究了转录组和基因组水平的代谢异常。
结果：从转录组测序数据表征的三个 hLCCL 亚组在各种代谢过程中表现出显着不同的畸变，包括氨基酸、脂质、能量和碳水化合物代谢。此外，全外显子组测序揭示了不同 hLCCL 亚组之间的不同突变特征，并确定了总共 19 个与代谢有关的已知驱动基因。
结论：我们的研究结果突出了肝癌发展过程中的不同代谢机制，并为进一步研究其代谢机制提供了资源。

关键词：人肝癌细胞系（hLCCLs），转录组，全外显子组，测序，肿瘤代谢