Abstract

Chinese herbal compound preparations are commonly composed of multiple traditional Chinese medicines (TCMs). The components of Chinese herbal compound preparation are complex, therefore, in this paper, ballpoint connector supported solvent microextraction (BC-SME) coupled with high-performance liquid chromatography with ultraviolet detection (HPLC/UV) is reported for the simultaneous extraction, enrichment and determination of the active flavonoids and anthraquinones with minimal waste production. The primary parameters affecting the performance were optimized, including the reuse of the ballpoint connector (BC), extraction solvent, pH of the sample solution, stirring rate, salt concentration, and extraction time. Under the optimal conditions, good linearities with r ≥ 0.9966 were obtained. The limits of detection (LODs) and quantitation (LOQs) were from 0.1 to 0.3 ng/mL and 0.4 to 0.9 ng/mL, respectively. The intra-day and inter-day relative standard deviations (RSDs) were less than 6.6% and the average recoveries were between 93.4% and 106.0%. Moreover, the analyte enrichment factors (EFs) were from 63 to 391. The results showed the method was simple, economical, sensitive, and provided high enrichment and allowed the extraction of flavonoids and anthraquinones in Chinese herbal compound preparations.

摘要

中草药复方制剂通常由多种中药组成。中草药复方制剂成分复杂，因此，本文报道了圆珠连接器支持的溶剂微萃取（BC-SME）与紫外检测高效液相色谱（HPLC/UV）联用，用于同时提取、富集和以最少的废物产生测定活性黄酮类化合物和蒽醌。对影响性能的主要参数进行了优化，包括圆珠笔连接器 (BC) 的重复使用、提取溶剂、样品溶液的 pH 值、搅拌速率、盐浓度和提取时间。在最佳条件下，与r 的良好线性获得≥0.9966。检测限 (LOD) 和定量 (LOQ) 分别为 0.1 至 0.3 ng/mL 和 0.4 至 0.9 ng/mL。日内和日间相对标准偏差 (RSD) 小于 6.6%，平均回收率在 93.4% 至 106.0% 之间。此外，分析物富集因子（EF s）为63～391。结果表明该方法简单、经济、灵敏，富集度高，可用于提取中草药复方制剂中的黄酮类和蒽醌类化合物。