To expand the bioimaging applications of Au and black phosphorus, black phosphorus quantum dot-Au hybrids (Au-BPQD nanohybrids) are fabricated for multiple theranostic applications in this study. By simply varying the ratios of BPQDs and Au³⁺, the BPQDs assisted Au³⁺ reduction outputs two kinds of nanohybrids with different functions. The less BPQDs lead to small size Au-BPQD nanohybrids (S-ABPs) with a core-shell structure. S-ABPs exhibit superior near-infrared (NIR) surface-enhanced Raman scattering (SERS) activity for the fingerprint analysis of biomolecules. It's also very promising for interference-free bioimaging due to the distinctive Raman band at around 2190 cm⁻¹ in the biologically Raman-silent region, which cannot be achieved by Au or BP-based nanomaterials. Meanwhile, further addition of BPQDs would lead to large size Au-BPQD nanohybrids (L-ABPs) with strong NIR optical absorption, which facilitates the in vivo 3D photoacoustic imaging as well as the photothermal therapy against tumor. This work reports versatile nanotheranostic platforms for amphibious SERS spectroscopy, 3D photoacoustic imaging and cancer therapy, which opens a new door for the biomedical applications of BP and Au based nanomaterials.

为了扩大 Au 和黑磷的生物成像应用，本研究中制造了用于多种治疗诊断应用的黑磷量子点-Au 杂化物（Au-BPQD 纳米杂化物）。通过简单地改变BPQDs和Au ³⁺的比例，BPQDs辅助Au ³⁺还原输出两种不同功能的纳米杂化物。较少的 BPQD 导致具有核壳结构的小尺寸 Au-BPQD 纳米杂化物 (S-ABP)。S-ABP 表现出优越的近红外 (NIR) 表面增强拉曼散射 (SERS) 活性，可用于生物分子的指纹分析。由于在 2190 cm ^-1附近的独特拉曼波段，它也非常有希望用于无干扰生物成像在生物拉曼静音区域，这是 Au 或 BP 基纳米材料无法实现的。同时，BPQDs的进一步添加将导致具有强近红外光吸收的大尺寸Au-BPQD纳米杂化物（L-ABPs），这有助于体内3D光声成像以及针对肿瘤的光热治疗。这项工作报告了用于两栖 SERS 光谱、3D 光声成像和癌症治疗的多功能纳米治疗平台，为 BP 和 Au 基纳米材料的生物医学应用打开了新的大门。