Previous studies have shown that blockers of voltage-gated potassium (Kv) channels, such as 4-aminopyridine (4-AP) and 2-methoxy-N-((1-phenylcyclopentyl)methyl)benzamide (2-MPB) synergized pyrethroid toxicity as well, or better than, piperonyl butoxide. The present study assessed the involvement of different Kv channels as possible pyrethroid synergist targets in Drosophila melanogaster. Three Kv1 mutants (Sh⁵, Sh¹³³, and Sh^M) and one Kv2 mutant (Shab³) were tested. All Kv1 mutant flies showed increased sensitivity to permethrin in topical and glass contact toxicity assays, of 2- to 11-fold. Central nervous system (CNS) recordings of larval D. melanogaster showed a similar pattern of increased sensitivity. Potentiated effects were also observed with deltamethrin on the mutants Sh⁵ (30- to 35-fold) and Sh¹³³ (33- to 47-fold), but the mutant Sh^M showed little change in sensitivity. In contrast, the Shab³ strain showed toxicity and physiological effects of both pyrethroids that were similar to the susceptible OR strain. Thus, some K⁺ channel mutations mimicked the synergistic effect of channel blockers. Additional studies showed that Shab³ had the highest sensitivity to 4-AP in topical assays, and the Shaker-null mutants, Sh^M and Sh¹³³ showed greater sensitivity to 2-MPB in CNS recordings of larval D. melanogaster. These results suggest that Kv1 channels are a useful synergist target for pyrethroids, as assessed both in whole insects and at the level of the nervous system. Thus, Kv1-targeting compounds can potentially serve as insect control tools to reduce pyrethroid use via synergistic action.

以前的研究表明，电压门控钾 (Kv) 通道的阻滞剂，如 4-氨基吡啶 (4-AP) 和 2-甲氧基-N -((1-苯基环戊基)甲基)苯甲酰胺 (2-MPB) 协同拟除虫菊酯毒性以及或优于胡椒基丁醚。本研究评估了不同 Kv 通道作为黑腹果蝇中可能的拟除虫菊酯增效剂目标的参与。测试了三个 Kv1 突变体（Sh ⁵、Sh ¹³³和 Sh ^M）和一个 Kv2 突变体（Shab ³）。在局部和玻璃接触毒性试验中，所有 Kv1 突变果蝇对氯菊酯的敏感性增加了 2 到 11 倍。黑腹果蝇幼虫的中枢神经系统 (CNS) 记录表现出类似的敏感性增加模式。还观察到溴氰菊酯对突变体 Sh ⁵（30 至 35 倍）和 Sh ¹³³（33 至 47 倍）的增强作用，但突变体 Sh ^{M 的}敏感性几乎没有变化。相比之下，Shab ³菌株显示出与敏感 OR 菌株相似的两种拟除虫菊酯的毒性和生理作用。因此，一些 K ⁺通道突变模仿了通道阻滞剂的协同作用。其他研究表明，Shab ³在局部检测中对 4-AP 的敏感性最高，而 Shaker-null 突变体 Sh ^M和 Sh ¹³³在黑腹果蝇幼虫的 CNS 记录中对 2-MPB 表现出更高的敏感性。这些结果表明 Kv1 通道是拟除虫菊酯的有用协同目标，正如在整个昆虫和神经系统水平上所评估的那样。因此，靶向 Kv1 的化合物可以作为昆虫控制工具，通过协同作用减少拟除虫菊酯的使用。