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Glycopatterns of the foregut in the striped dolphin Stenella coeruleoalba, Meyen 1833 from the Mediterranean Sea
Marine Mammal Science ( IF 2.3 ) Pub Date : 2021-07-23 , DOI: 10.1111/mms.12852
Roberto Carlucci 1 , Giulia Cipriano 1 , Carmelo Fanizza 2 , Tommaso Gerussi 3 , Rosalia Maglietta 4 , Antonio Petrella 5 , Guido Pietroluongo 3 , Pasquale Ricci 1 , Daniela Semeraro 1 , Marco Vito Guglielmi 1 , Giovanni Scillitani 1 , Donatella Mentino 6
Affiliation  

The glycopatterns of the glycans secreted by the mucosa of stomach and duodenal ampulla of the striped dolphin, Stenella coeruleoalba were studied by histochemical (Periodic acid-Schiff, Alcian Blue pH 2.5, High Iron Diamine) and lectin-binding (SBA, DBA, PNA, WGA, MAA-II, SNA, ConA, UEA-I, AAA, LTA) techniques. The stomach can be divided into four compartments: main stomach, two connecting chambers and pylorus. The pylorus is followed by the duodenal ampulla. Mucins are secreted by surface cells and intramucosal glands specific for each compartment. In the main stomach glands, neck cells were weakly sulphated, with prevailing glycosaminylated, glycosylated/mannosylated, and fucosylated residuals. Parietal and chief cells in general were scarcely reactive. In the connecting chambers glands, there were high levels of sulphation, glycosaminylation, glycosylation/mannosylation, and fucosylation, the latter with more complex patterns than those observed in the main stomach glands. In the pyloric glands sulphated, glycosaminylated and fucosylated residuals decreased, whereas the opposite was observed for galactosyl/galactosaminylated residuals. Glycosylation patterns in the glands of the duodenal ampulla differed from those of the pyloric ones, with similar levels of sulphation, lower levels of galactosyl/galactosaminylation and glycosaminylation, and higher level of fucosylation. The results are compared with those available in literature.

中文翻译:

来自地中海的 Meyen 1833 条纹海豚 Stenella coeruleoalba 的前肠糖型

条纹海豚Stenella coeruleoalba胃粘膜和十二指肠壶腹分泌的聚糖的糖型通过组织化学(过碘酸-希夫、阿尔新蓝 pH 2.5、高铁二胺)和凝集素结合(SBA、DBA、PNA、WGA、MAA-II、SNA、ConA、UEA-I、AAA、LTA)技术进行了研究。胃可分为四个隔室:主胃、两个连接腔和幽门。幽门之后是十二指肠壶腹。粘蛋白由每个隔室特异性的表面细胞和粘膜内腺体分泌。在主要胃腺中,颈部细胞被弱硫酸化,糖胺基化、糖基化/甘露糖基化和岩藻糖基化残留物占主导地位。壁细胞和主细胞一般几乎没有反应。在连接腔腺体中,存在高水平的硫酸化、糖胺化、糖基化/甘露糖基化和岩藻糖基化,后者的模式比在主要胃腺中观察到的更复杂。在硫酸化的幽门腺中,糖胺化和岩藻糖基化的残留物减少,而对于半乳糖基/半乳糖胺化的残留物观察到相反的情况。十二指肠壶腹腺体的糖基化模式与幽门腺体不同,硫酸化水平相似,半乳糖基/半乳糖胺基化和糖胺基化水平较低,岩藻糖基化水平较高。结果与文献中的结果进行了比较。和更高水平的岩藻糖基化。结果与文献中的结果进行了比较。和更高水平的岩藻糖基化。结果与文献中的结果进行了比较。
更新日期:2021-07-23
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