Polydiacetylene (PDA) vesicles provide useful stimuli-responsive behavior as well as by the modular structure afford a means for the design of sensing and delivery systems with tunable target specificity. To reduce inherent non-specific interaction with either anionic or cationic formulations of polydiacetylene vesicles, we explored the use of various lengths of poly(ethylene glycol) (PEG) amphiphiles for integration and polymerization within PDA vesicles. Our results established that as little as 1% of polyethylene glycol amphiphile integration into anionic vesicles was sufficient to significantly reduce non-specific association with mammalian cells. Similarly integrating a low percent of PEG amphiphile content within cationic vesicles could also significantly reduce non-specific cell association, and moreover reduced cytotoxicity. These results may be prove useful in augmenting PDA vesicles formulations for reduced non-specific interaction which is of particularly interest to enhancing selectivity in vesicles designed with integrated targeting moieties for sensing and drug delivery applications.

聚二乙炔 (PDA) 囊泡提供有用的刺激响应行为，并且通过模块化结构提供了一种用于设计具有可调目标特异性的传感和递送系统的方法。为了减少与聚二乙炔囊泡的阴离子或阳离子配方的固有非特异性相互作用，我们探索了使用各种长度的聚（乙二醇）（PEG）两亲物在 PDA 囊泡内进行整合和聚合。我们的研究结果表明，低至 1% 的聚乙二醇两亲物整合到阴离子囊泡中就足以显着减少与哺乳动物细胞的非特异性结合。类似地，在阳离子囊泡中整合低百分比的 PEG 两亲物含量也可以显着降低非特异性细胞结合，此外还可以降低细胞毒性。