Two new ecdysteroids 14-epi-polypodine B (1) and 22-oxo-hydroxyecdysterone (2), along with nine known compounds, polypodine B (3), viticosterone E (4), 20-hdroxyecdysone-2-acetate (5), 22-oxo-20-hydroxyecdysone (6), 5-hydroxyecdysone (7), pinnatasterone (8), 3-epi-20-hydroxyecdysone (9), ecdysterone (10) and stachysterone B (11), were isolated from the aerial parts of Paris verticillata. The structures of all compounds were elucidated by extensive spectroscopic analysis, quantum chemical calculations and ANN-PRA/DP4⁺ probability analysis. Among them, the absolute configuration of compound 1 and 2 was unambiguous determined by ECD. Also, the isolated compounds were assessed for their cytotoxic activities. Compounds 2, 3 and 7 exhibited significant cytotoxic activities against PC12, LN299 and SMCC7721 cells.

中文翻译：

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来自Paris verticillata空中部分的蜕皮激素

两种新的蜕皮激素 14-epi-polypodine B ( 1 ) 和 22-oxo-hydroxyecdysterone ( 2 )，以及九种已知化合物，polypodine B ( 3 )、viticosterone E ( 4 )、20-hroxyecdysone-2-acetate ( 5 ) , 22-oxo-20-羟基蜕皮激素( 6 ), 5-羟基蜕皮激素 ( 7 ), pinnatasterone ( 8 ), 3-epi-20-hydroxyecdysone ( 9 ), ecdysterone ( 10 ) and stachysterone B ( 11 ), 从Paris verticillata 的地上部分。通过广泛的光谱分析、量子化学计算和 ANN-PRA/DP4 ⁺阐明了所有化合物的结构概率分析。其中，化合物1和2的绝对构型由 ECD 确定。此外，还评估了分离的化合物的细胞毒活性。化合物2，3和7表现出对PC12，LN299和SMCC7721细胞显著细胞毒性活性。