Ubiquitination plays a prominent role in cell signaling networks. Enzymes governing ubiquitination include the conjugation machinery along with the deconjugating enzymes - deubiquitases. A MspA nanopore is demonstrated as a sensor for discriminating and quantifying various ubiquitin proteins. Real-time measurement of polymeric ubiquitin chain disassembly by a deubiquitinating enzyme was performed and enzyme kinetics were determined. The implementation of MspA into arrays could enable high throughput characterizations of unknown deubiquitinases as well as drug screening against disease-related enzymes. More information can be found in the Communication by Min Chen et al.

中文翻译：

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封面特征：使用 MspA 纳米孔实时无标记地测量去泛素化酶活性 (ChemBioChem 17/2021)

泛素化在细胞信号网络中发挥着重要作用。控制泛素化的酶包括结合机制以及去结合酶 - 去泛素化酶。MspA 纳米孔被证明是一种用于区分和量化各种泛素蛋白的传感器。进行了去泛素化酶对聚合泛素链分解的实时测量，并确定了酶动力学。在阵列中实施 MspA 可以实现未知去泛素化酶的高通量表征以及针对疾病相关酶的药物筛选。更多信息可以在 Min Chen 等人的通讯中找到。