Liquid-phase electron microscopy (LP-EM) is an exciting new area in the materials imaging field, providing unprecedented views of molecular processes. Time-resolved insights from LP-EM studies are a strong complement to the remarkable results achievable with other high-resolution techniques. Here, the opportunities to expand LP-EM technology beyond 2D temporal assessments and into the 3D regime are described. The results show new structures and dynamic insights of human viruses contained in minute volumes of liquid while acquired in a rapid timeframe. To develop this strategy, adeno-associated virus (AAV) is used as a model system. AAV is a well-known gene therapy vehicle with current applications involving drug delivery and vaccine development for COVID-19. Improving the understanding of the physical properties of biological entities in a liquid state, as maintained in the human body, has broad societal implications for human health and disease.

中文翻译：

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液滴中人类病毒的高分辨率成像

液相电子显微镜（LP-EM）是材料成像领域一个令人兴奋的新领域，提供了前所未有的分子过程视图。LP-EM 研究的时间分辨见解是对其他高分辨率技术可实现的显着结果的有力补充。这里描述了将 LP-EM 技术扩展到 2D 时间评估之外并进入 3D 领域的机会。结果显示了在快速时间内获得的微量液体中包含的人类病毒的新结构和动态见解。为了开发这一策略，使用腺相关病毒（AAV）作为模型系统。AAV 是一种著名的基因治疗工具，目前的应用涉及 COVID-19 的药物输送和疫苗开发。提高对人体内液态生物实体物理特性的理解，对人类健康和疾病具有广泛的社会影响。