Severe leaf curl disease of tomato (ToLCD) was noticed recently in the central parts of India and is an emerging threat to the cultivation of tomato. The genomic components of the begomovirus isolate, DNA A and betasatellite associated with ToLCD were cloned by rolling circle amplification method and sequenced. The sequence analysis revealed that the DNA A (2766 nt) of this isolate had the nucleotide identity of >91% with other strains of Tomato leaf curl Karnataka virus (ToLCKV), hence this isolate is proposed as a strain of ToLCKV, named as ToLCKV-Raipur. Similarly, the betasatellite molecule (1355 nt) had the highest identity of 91.1% with Corchorus yellow vein mosaic betasatellite (CoYVMB) and named as CoYVMB-Raipur. The full-length dimerized clones of these two genomic components were agroinoculated on natural (tomato), experimental (Nicotiana benthamiana) hosts and other 20 plant species belong to six different families. The severe leaf curl symptoms appeared only in the hosts, N. benthamiana, and in tomato inoculated with ToLCKV-Raipur alone and ToLCKV-Raipur with CoYVMB-Raipur after 8 and 16-18 days inoculation, respectively. This isolate was also transmissible to healthy tomato plants by whitefly from the tomato plant agroinoculated with ToLCKV-Raipur alone and with CoYVMB-Raipur and produced symptoms within 14-16 days after inoculation. Interestingly, this isolate infects horse gram and chilli by whitefly transmission and both the hosts showed positive for DNA A alone but not for betasatellite. Quantification of the genomic components of this isolate with the agroinoculated N. benthamiana samples by qRT-PCR results showed that the quantity of ToLCKV-Raipur was enhanced by three-fold while inoculated with CoYVMB-Raipur compared to ToLCKV-Raipur alone inoculated plants. However, CoYVMB-Raipur did not enhance the levels of ToLCKV-Raipur in the agroinoculated tomato plants. This is the first evidence of the natural co-occurrence of ToLCKV with betasatellite, CoYVMB causing ToLCD.

最近在印度中部地区发现了严重的番茄卷叶病（ToLCD），这是对番茄种植的新威胁。Begomovirus 分离物、DNA A 和与 ToLCD 相关的 β 卫星的基因组成分通过滚环扩增法克隆并测序。序列分析表明，该分离株的DNA A（2766 nt）与其他番茄卷叶卡纳塔克病毒（ToLCKV）株的核苷酸同一性>91%，因此该分离株被提出为ToLCKV株，命名为ToLCKV -赖布尔。同样，β 卫星分子 (1355 nt) 与Corchorus 黄脉镶嵌β卫星的同一性最高，为 91.1%(CoYVMB) 并命名为 CoYVMB-Raipur。将这两个基因组成分的全长二聚体克隆接种到自然（番茄）、实验（本氏烟草）宿主和属于六个不同科的其他 20 种植物物种上。严重的卷叶症状仅出现在宿主本氏烟草中，分别在接种 8 天和 16-18 天后单独接种 ToLCKV-Raipur 和接种 CoYVMB-Raipur 的 ToLCKV-Raipur 的番茄中。这种分离物也可以通过粉虱从单独用 ToLCKV-Raipur 和 CoYVMB-Raipur 农杆菌接种的番茄植物中传播给健康的番茄植物，并在接种后 14-16 天内产生症状。有趣的是，这种分离株通过粉虱传播感染了马革兰和辣椒，并且两个宿主都显示出单独的 DNA A 阳性，而不是 β 卫星。用农杆菌接种的本氏烟草对这种分离物的基因组成分进行定量qRT-PCR 的样本结果表明，与单独接种 ToLCKV-Raipur 的植物相比，接种 CoYVMB-Raipur 的 ToLCKV-Raipur 的数量增加了三倍。然而，CoYVMB-Raipur 并没有提高农杆菌接种番茄植株中 ToLCKV-Raipur 的水平。这是 ToLCKV 与 beta 卫星自然共现的第一个证据，CoYVMB 导致 ToLCD。