Herein, a two-dimensional (2D) Co(OH)₂ nanosheet (NS) was prepared by reacting Co(II) acetate with 2-ethylimidazole and trimethylamine at room temperature. The as-prepared Co(OH)₂ NS-modified screen-printed electrode enabled glucose sensing in human blood plasma samples (HBP) diluted with phosphate buffer saline (PBS, pH 7.4) and NaOH (0.1 M, pH 13.0) solutions. The electrocatalytic activity of Co(OH)₂ NS for glucose oxidation in a wide pH range can be ascribed to the enriched oxygen vacancies with a high surface area of the porous NS, which enabled the facile formation of Co^II/Co^III redox pair. The oxidation current response as a function of glucose concentration displayed two straight lines in the ranges 55.12–117.50 μM and 180–12055 μM in PBS diluted HBP, and 62.80–180 μM and 305–12055 μM in NaOH diluted HBP with the sensitivity of 430 and 98.18 μA/cm²/mM and the limit of detection of 0.082 and 2.2 μM, in PBS and NaOH diluted HBP solutions, respectively. The sensor also exhibited good long-term stability, repeatability, and interference-free signals. The good analytical performance of the proposed sensor demonstrates the suitability of Co(OH)₂ NS as a material to fabricate disposable systems for the direct detection of glucose in human body fluids at physiological pH.

在此，二维 (2D) Co(OH) ₂纳米片 (NS) 通过醋酸 Co(II) 与 2-乙基咪唑和三甲胺在室温下反应制备。所制备的 Co(OH) ₂ NS 修饰的丝网印刷电极能够在用磷酸盐缓冲盐水 (PBS, pH 7.4) 和 NaOH (0.1 M, pH 13.0) 溶液稀释的人血浆样品 (HBP) 中检测葡萄糖。Co(OH) ₂ NS在较宽的pH范围内对葡萄糖氧化的电催化活性可归因于多孔NS具有高表面积的富氧空位，这使得Co ^II / Co ^III易于形成氧化还原对。作为葡萄糖浓度函数的氧化电流响应在 PBS 稀释的 HBP 中显示出 55.12-117.50 μM 和 180-12055 μM 范围内的两条直线，在 NaOH 稀释的 HBP 中显示 62.80-180 μM 和 305-12055 μM，灵敏度为 430和 98.18 μA/cm ² /mM，检测限分别为 0.082 和 2.2 μM，在 PBS 和 NaOH 稀释的 HBP 溶液中。该传感器还表现出良好的长期稳定性、可重复性和无干扰信号。所提出的传感器的良好分析性能证明了 Co(OH) ₂ NS 作为制造一次性系统的材料的适用性，该系统用于在生理 pH 值下直接检测人体体液中的葡萄糖。