Climate changes are promoting increases in global temperatures, variability in precipitation, and more frequent insect pest outbreaks. Understanding the molecular mechanisms for the tolerance of both, biotic and abiotic stresses, is critical for the development of improved genotypes and sustainability of productivity under climate change. Thus, we examine the regulatory cascades and metabolic pathways triggered by both drought and insect attack signals. Profiles from the genotypes drought-tolerant (EMBRAPA 48) and resistant to A. gemmatalis infestation (IAC17) were compared with one sensitive to drought/insect attack (BR16). EMBRAPA 48 and IAC17 plants under drought were less susceptible to insect attack, promoting lower caterpillar survival. Survival reductions were not dependent on the drought-tolerance or insect-resistance phenotypes, despite being more pronounced for IAC17. Furthermore, metabolites profiles, gene expression and enzymatic assays lead us to conclude that drought signal by itself was not enough to explain the survival reductions. Protease inhibition activities and expressions of LOX and PI genes correlated with ABA levels, indicating that JA-signalling was potentialized by ABA to enhance the production of deterrent metabolites. Thus, increased ABA levels during drought treatment may be acting synergistically to induce cascades responsive to JA. Thus, regulatory molecular hub(s) integrating signals may be a target to the genetic engineering of plants with multiple tolerances to environmental stresses.

气候变化正在促进全球气温升高、降水变化和更频繁的虫害爆发。了解生物和非生物胁迫耐受的分子机制对于气候变化下改良基因型的开发和生产力的可持续性至关重要。因此，我们研究了由干旱和昆虫攻击信号触发的调节级联和代谢途径。来自耐旱 (EMBRAPA 48) 和抗A. gemmatalis 基因型的概况侵染 (IAC17) 与对干旱/昆虫侵袭敏感 (BR16) 进行了比较。干旱条件下的 EMBRAPA 48 和 IAC17 植物不太容易受到昆虫侵袭，从而降低了毛毛虫的存活率。尽管 IAC17 的存活率降低更明显，但不依赖于耐旱性或抗虫性表型。此外，代谢物谱、基因表达和酶促测定使我们得出结论，干旱信号本身不足以解释存活率降低。蛋白酶抑制活性以及 LOX 和 PI 基因的表达与 ABA 水平相关，表明 ABA 可能通过 JA 信号来增强威慑代谢物的产生。因此，干旱处理期间增加的 ABA 水平可能协同作用以诱导响应 JA 的级联反应。因此，