Pure titanium alloys are commonly employed in construction of dental implants, given their advantageous features including resistance to corrosion, mechanical strength, flexibility, and bioavailability. The purpose of this study was to evaluate the surface characteristics of Ti6Al4V alloy disks upon sand-blasted, large-grit acid-etching, and/or selective laser melting applications and to assess whether and how these surface modifications affect the attachment, viability, metabolic activity, and osteoblastic differentiation of Saos-2 osteosarcoma cells. We manufactured Ti6Al4V alloy disks and divided them into four groups (non-treated, SLA-treated, Ti6Al4V ELI alloy-coated (SLM treated), and SLM+SLA-treated). The topographic analysis was carried using AFM and SEM and chemical content was evaluated by EDX. EDS. Adherence and viability of Saos-2 cells seeded onto disks were investigated via SEM and fluorescent microscopy. To assess the metabolic activity of Saos-2 cells, MTT assay was conducted and the osteoblastic differentiation interpreted via monitoring alkaline phosphatase activity. According to data acquired using AFM and SEM, the control group had the smoothest surfaces, with a lower Ra value. The roughest surface was obtained with SLM and SLA dual-treated disks. The osteoblastic activity of Saos-2 cells on the surface of dual-treated disks was higher than the other groups. Therefore, the surface of SLM+SLA-treated disks conferred a suitable environment for Saos-2 cells to adhere, proliferate, and show higher metabolic activity. We concluded that Ti6Al4V alloy disks covered with Ti6Al4V ELI alloy and subjected to SLA surface treatment may be promising to manufacture dental implants with improved adaptability, bioavailability, and osseointegration.

纯钛合金通常用于制造牙科植入物，因为它们具有抗腐蚀、机械强度、柔韧性和生物利用度等优点。本研究的目的是评估 Ti6Al4V 合金盘在喷砂、大粒度酸蚀刻和/或选择性激光熔化应用中的表面特性，并评估这些表面改性是否以及如何影响附着、活力、代谢Saos-2 骨肉瘤细胞的活性和成骨细胞分化。我们制造了 Ti6Al4V 合金盘并将它们分为四组（未处理、SLA 处理、Ti6Al4V ELI 合金涂层（SLM 处理）和 SLM+SLA 处理）。使用 AFM 和 SEM 进行形貌分析，并通过 EDX 评估化学含量。电子数据系统。通过扫描电镜和荧光显微镜研究接种到圆盘上的 Saos-2 细胞的粘附性和活力。为了评估 Saos-2 细胞的代谢活性，进行了 MTT 测定，并通过监测碱性磷酸酶活性来解释成骨细胞分化。根据使用 AFM 和 SEM 获得的数据，对照组具有最光滑的表面，具有较低的 Ra 值。最粗糙的表面是用 SLM 和 SLA 双重处理的磁盘获得的。Saos-2细胞在双处理椎间盘表面的成骨细胞活性高于其他组。因此，SLM+SLA 处理的圆盘表面为 Saos-2 细胞提供了一个合适的环境，使其能够粘附、增殖并表现出更高的代谢活性。