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Clostridium culturomicium sp. nov. and Clostridium jeddahitimonense sp. nov., novel members of the Clostridium genus isolated from the stool of an obese Saudi Arabian
Current Microbiology ( IF 2.6 ) Pub Date : 2021-07-23 , DOI: 10.1007/s00284-021-02616-4
Hoang Thong Kieu 1, 2 , Noémie Garrigou 2 , Amael Fadlane 2 , Ludivine Brechard 2 , Nicholas Armstrong 2 , Philippe Decloquement 2 , Muhammad Yasir 3 , Esam Ibraheem Azhar 3, 4 , Saad B Al-Masaudi 5 , Jean-Christophe Lagier 1, 2 , Maryam Tidjani Alou 1 , Didier Raoult 1, 2
Affiliation  

Taxono-genomics is an innovative concept coined for the description of new bacterial species. Phenotypic characteristics were combined with a genomic approach to describe two new species within the Clostridium senso stricto genus: Clostridium culturomicium strain CL-6T and Clostridium jeddahitimonense strain CL-2T, both isolated from the gut microbiota of an obese man from Saudi Arabia. Strains CL-6T and CL-2T shared a similarity of 98.4% with the 16S rRNA gene of Clostridium subterminale strain JCM 1417T (accession number NR113027) and 98% with that of Clostridium disporicum strain DS1T (accession number NR026491), respectively. The highest OrthoANI values were shared with Clostridium punense for strain CL-6T (70.8%) and with Clostridium disporicum for strain CL-2T (87.1%). Additionally, strain CL-6T and strain CL-2T shared a 16S rRNA similarity of 91.4%. Both strains were anaerobic, spore-forming and Gram-stain-positive non-motile bacilli. The genome of Clostridium culturomicium strain CL-6T is 4,325,182 bp long with 32.2% GC content. As for Clostridium jeddahitimonense strain CL-2T, the genome is 4,074,758 bp long with 29.2% GC content.



中文翻译:

Clostridium culturomicium sp. 十一月 和 Clostridium jeddahitimonense sp。11 月,从一个肥胖的沙特阿拉伯人的粪便中分离出梭菌属的新成员

分类基因组学是一个创新概念,用于描述新的细菌物种。将表型特征与基因组方法相结合,描述了狭义梭菌属内的两个新物种:培养梭菌CL-6 T吉达希莫梭菌CL-2 T菌株,两者均从沙特阿拉伯肥胖男性的肠道微生物群中分离出来。菌株CL-6 Ť和CL-2 Ť共享98.4%的相似性与16S rRNA基因近端梭菌菌株JCM 1417 Ť(登录号NR113027)和98%与的梭菌disporicum菌株 DS1 T(登录号 NR026491),分别。最高的 OrthoANI 值与Clostridium punense菌株 CL-6 T (70.8%) 和Clostridium disporicum菌株 CL-2 T (87.1%)共享。此外,菌株 CL-6 T和菌株 CL-2 T具有 91.4% 的 16S rRNA 相似性。两种菌株都是厌氧的、形成孢子的和革兰氏染色阳性的非运动性杆菌。Clostridium culturomicium菌株 CL-6 T的基因组长4,325,182 bp,GC 含量为 32.2%。至于Clostridium jeddahitimonense菌株 CL-2 T,基因组长 4,074,758 bp,GC 含量为 29.2%。

更新日期:2021-08-19
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