Abstract

Florfenicol has been widely used in livestock and aquaculture to treat bacterial infections. Due to the abuse of antibiotics, florfenicol has been released into the environment. Thus, there is a need for the rapid determination of trace levels of florfenicol in the environment. To improve the sensitivity for florfenicol, solid phase extraction (SPE) was employed to enrich florfenicol from multi-residue antibiotic mixtures followed by in situ determination by surface-enhanced Raman spectroscopy (SERS). Activated carbon, C₁₈, graphene oxide (GO), and sand were applied to concentrate florfenicol in water. Silver nanoparticles (AgNP)-coated silicon (AgNPs@Si) substrate was employed to determine florfenicol enriched on the adsorbent. The results showed that the detection limit of florfenicol in water was 2.8 × 10⁻⁹mol/L following the adsorption and enrichment of activated carbon. Besides, activated carbon was performed to extract florfenicol from the multi-residue antibiotic mixtures, including enrofloxacin, norfloxacin, and florfenicol. The results showed that activated carbon combined with SERS specifically determined florfenicol in complex antibiotic mixtures with a detection limit of 2.8 × 10⁻⁹mol/L. Moreover, to better understand the adsorption mechanisms, the mechanism of the interaction of florfenicol with activated carbon was investigated.

摘要

氟苯尼考已广泛用于牲畜和水产养殖中以治疗细菌感染。由于滥用抗生素，氟苯尼考已被释放到环境中。因此，需要快速测定环境中的痕量氟苯尼考。为了提高氟苯尼考的​​灵敏度，采用固相萃取 (SPE) 从多残留抗生素混合物中富集氟苯尼考，然后通过表面增强拉曼光谱 (SERS) 进行原位测定。活性炭、C ₁₈、氧化石墨烯 (GO) 和沙子用于浓缩水中的氟苯尼考。银纳米粒子 (AgNP) 涂层的硅 (AgNPs@Si) 基板用于测定吸附剂上富集的氟苯尼考。结果表明，氟苯尼考在水中的检出限为2.8×10^-9 mol/L 在活性炭的吸附和富集之后。此外，使用活性炭从多残留抗生素混合物中提取氟苯尼考，包括恩诺沙星、诺氟沙星和氟苯尼考。结果表明，活性炭与 SERS 结合可特异性测定复杂抗生素混合物中的氟苯尼考，检测限为 2.8 × 10 ^-9 mol/L。此外，为了更好地了解吸附机制，研究了氟苯尼考与活性炭相互作用的机制。