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The effects of despeckling filters on pore size measurements in collagen scaffold micro-CT data
Journal of Microscopy ( IF 2 ) Pub Date : 2021-07-22 , DOI: 10.1111/jmi.13050
Matt C Meek 1 , Serena Best 1 , Ruth Cameron 1
Affiliation  

Micro-CT is often used to assess the characteristics of porous structures such as tissue engineering scaffolds and trabecular bone. Prior to analysis, micro-CT images can be thresholded and filtered to remove noise. Scaffold pore size affects mechanical properties and biological cell behaviour and is a frequently assessed parameter. This paper identifies and characterizes an artefact affecting a commonly used filter which erroneously increases mean pore size. The 3D sweep despeckling filter removes all but the largest object within a volume of interest, and therefore deletes any disconnected objects located at the periphery, increasing measured mean pore size. This artefact is characterized, and effective methods to mitigate its effects are devised, involving despeckling a sufficiently large volume of interest, then reducing the volume of interest in size to remove the error prior to analysis. Techniques to effectively apply this method to other data sets are described. This method eliminates the artefact but is time-consuming and computationally expensive. Alternative, more economical filters which remove objects below a specified size are also assessed but are shown to be affected by the same artefact. These results will help to guide the implementation of future studies investigating the effects of pore size.

中文翻译:

去斑过滤器对胶原支架微 CT 数据中孔径测量的影响

Micro-CT 常用于评估多孔结构的特性,如组织工程支架和骨小梁。在分析之前,可以对显微 CT 图像进行阈值处理和过滤以去除噪声。支架孔径影响机械性能和生物细胞行为,是经常评估的参数。本文识别并表征了影响常用过滤器的人工制品,该过滤器错误地增加了平均孔径。3D 扫描去斑过滤器会移除感兴趣体积内除最大对象之外的所有对象,因此会删除位于外围的任何不连接对象,从而增加测量的平均孔径。该人工制品已被表征,并设计了减轻其影响的有效方法,包括去除足够大的感兴趣体积,然后在分析之前减少感兴趣的体积的大小以消除错误。描述了将这种方法有效应用于其他数据集的技术。这种方法消除了人工制品,但耗时且计算成本高。还评估了可去除指定尺寸以下物体的替代性、更经济的过滤器,但表明会受到相同的人工制品的影响。这些结果将有助于指导未来调查孔径影响的研究的实施。还评估了去除低于指定尺寸的物体的更经济的过滤器,但表明会受到相同的人工制品的影响。这些结果将有助于指导未来调查孔径影响的研究的实施。还评估了去除低于指定尺寸的物体的更经济的过滤器,但表明会受到相同的人工制品的影响。这些结果将有助于指导未来调查孔径影响的研究的实施。
更新日期:2021-07-22
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