Advancements in chemical proteomics and mass spectrometry lipidomics are providing new opportunities to understand lipid kinase activity, specificity, and regulation on a global cellular scale. Here, we describe recent developments in chemical biology of lipid kinases with a focus on those members that phosphorylate diacylglycerols. We further discuss future implications of how these mass spectrometry–based approaches can be adapted for studies of additional lipid kinase members with the aim of bridging the gap between protein and lipid kinase–focused investigations.

中文翻译：

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脂质激酶的化学​​蛋白质组学评价进展——以DAG激酶为例

化学蛋白质组学和质谱脂质组学的进步为理解脂质激酶活性、特异性和全球细胞规模的调控提供了新的机会。在这里，我们描述了脂质激酶化学生物学的最新发展，重点是那些磷酸化甘油二酯的成员。我们进一步讨论了这些基于质谱的方法如何适用于其他脂质激酶成员研究的未来影响，目的是弥合以蛋白质和脂质激酶为重点的研究之间的差距。