Periodontal diseases can lead to soft tissue defects. Tissue engineering can provide functional replacements for damaged tissues. Recently, electrospun nanofibers have attracted great interest for tissue engineering and drug delivery applications. This has been revealed that statins exhibit positive impacts on the proliferation and regeneration of periodontal tissues. Electrospun simvastatin loaded poly (lactic-co-glycolic acid) (SIM-PLGA-NF) were prepared using electrospinning technique. Optimal conditions for preparation of SIM-PLGA-NF (PLGA concentration of 30 wt%, voltage of 15 kV, and flow rate of 1.5 ml h⁻¹) were identified using a 2³ factorial design. The optimized SIM-PLGA-NFs (diameter of 640.2 ± 32.5 nm and simvastatin entrapment efficacy of 99.6 ± 1.5%) were surface modified with 1% w/v hyaluronic acid solution (1%HA- SIM-PLGA-NF) to improve their compatibility with fibroblasts and potential application as a periodontal tissue engineering scaffold. HA-SIM-PLGA NFs were analyzed using SEM, FTIR, and XRD. 1%HA-SIM-PLGA-NF had uniform, bead-free and interwoven morphology, which is similar to the extracellular matrix. The mechanical performance of SIM-PLGA-NFs and release profile of simvastatin from these nanofibers have been also greatly improved after coating with HA. In vitro cellular tests showed that the proliferation, adhesion, and differentiation of fibroblast cells positively enhanced on the surface of 1%HA- SIM-PLGA-NF. These results demonstrate the potential application of 1%HA-SIM-PLGA-NFs as a scaffold for periodontal tissue engineering.

中文翻译：

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用于牙周组织工程的含辛伐他汀透明质酸包覆聚乳酸-乙醇酸共聚物纳米纤维的制备与表征

牙周病可导致软组织缺损。组织工程可以为受损组织提供功能替代品。最近，电纺纳米纤维在组织工程和药物输送应用中引起了极大的兴趣。这表明他汀类药物对牙周组织的增殖和再生具有积极影响。使用静电纺丝技术制备静电纺丝辛伐他汀负载聚（乳酸-共-乙醇酸）（SIM-PLGA-NF）。使用 2 ³确定制备 SIM-PLGA-NF 的最佳条件（PLGA 浓度为 30 wt%，电压为 15 kV，流速为 1.5 ml h ^{-1 ）}因子设计。用 1% w/v 透明质酸溶液 (1%HA-SIM-PLGA-NF) 对优化的 SIM-PLGA-NF（直径为 640.2 ± 32.5 nm，辛伐他汀包封率为 99.6 ± 1.5%）进行表面改性，以改善其与成纤维细胞的相容性和作为牙周组织工程支架的潜在应用。使用 SEM、FTIR 和 XRD 分析 HA-SIM-PLGA NF。1%HA-SIM-PLGA-NF具有均匀、无珠和交织的形态，与细胞外基质相似。SIM-PLGA-NFs 的机械性能和辛伐他汀从这些纳米纤维中的释放曲线在涂敷 HA 后也得到了极大的改善。体外细胞试验表明，1%HA-SIM-PLGA-NF表面成纤维细胞的增殖、粘附和分化呈正增强。