Abstract

This study was performed to investigate the effects of curcumin supplementation on intestinal barrier function, serum biochemical index, amino acid profile, and mRNA expression of nutrient transporters in laying hens fed soybean meal with heat-induced protein oxidation. A total of 288 40-week-old Hy-Line Brown laying hens were allocated into one of three treatments with eight replicates of twelve birds each for a 42-day feeding trial. The birds were fed a soybean meal-corn basal diet and a heated soybean meal (HSBM)-corn basal diet supplemented with or without 150 mg/kg curcumin. HSBM increased circulating diamine oxidase activity and D-lactate levels, and curcumin reduced D-lactate concentrations in birds fed HSBM (p < .05). Birds fed HSBM exhibited a decreased mRNA abundance of occludin and claudin 3 in the intestinal mucosa, and downregulation of claudin 3 expression was reversed by curcumin (p < .05). HSBM decreased total protein and albumin levels in the serum, both of these levels being increased with curcumin (p < .05). The administration of curcumin reversed HSBM-induced downregulation of amino acid concentrations in the serum, liver and yolk to control levels (p < .05). Moreover, HSBM decreased mRNA expression levels of amino acid transporters, glucose transporters, and peptide transporters in the intestinal mucosa, and curcumin almost completely reversed these effects, restoring expression levels of these genes to the control values (p < .05). The results of this study indicate that curcumin administration can alleviate the intestinal barrier injury and compromised nutrient absorption induced by HSBM in laying hens.

• HIGHLIGHTS

• Protein oxidation of heated soybean meal (HSBM) impaired the intestinal barrier function of laying hens.

• Curcumin treatment attenuated the intestinal barrier injury induced by HSBM.

• Curcumin treatment improved the reduced nutrient absorption and transport of hens fed HSBM.

摘要

本研究旨在研究补充姜黄素对饲喂热诱导蛋白质氧化豆粕的蛋鸡肠道屏障功能、血清生化指标、氨基酸谱和营养转运蛋白 mRNA 表达的影响。总共 288 只 40 周龄的 Hy-Line Brown 蛋鸡被分配到三个处理中的一个，每组 8 个重复，每组 12 只，用于 42 天的饲养试验。给这些鸡喂食豆粕-玉米基础日粮和加热豆粕 (HSBM)-玉米基础日粮，添加或不添加 150 mg/kg 姜黄素。HSBM 增加了循环二胺氧化酶活性和 D-乳酸水平，姜黄素降低了喂食 HSBM 的鸟类的 D-乳酸浓度（p < .05）。喂食 HSBM 的鸟类在肠粘膜中表现出 occludin 和 claudin 3 的 mRNA 丰度降低，并且姜黄素逆转了 claudin 3 表达的下调 ( p  < .05)。HSBM 降低了血清中的总蛋白和白蛋白水平，这两种水平都随着姜黄素而增加 ( p  < .05)。姜黄素的给药逆转了 HSBM 诱导的血清、肝脏和蛋黄中氨基酸浓度的下调至控制水平 ( p  < .05)。此外，HSBM 降低了肠黏膜中氨基酸转运蛋白、葡萄糖转运蛋白和肽转运蛋白的 mRNA 表达水平，姜黄素几乎完全逆转了这些影响，将这些基因的表达水平恢复到对照值（p < .05）。本研究的结果表明，姜黄素给药可以减轻 HSBM 引起的蛋鸡肠​​道屏障损伤和营养吸收受损。

• 强调

• 加热豆粕 (HSBM) 的蛋白质氧化损害了蛋鸡的肠道屏障功能。

• 姜黄素治疗减轻了 HSBM 诱导的肠道屏障损伤。

• 姜黄素处理改善了喂食 HSBM 的母鸡减少的营养吸收和运输。