In this study, molecular genetic diversity and phylogenetic analysis of Turkish (Aegean region) Punica granatum L. populations was conducted based on ISSR-PCR and chloroplast DNA trnL‑F sequences. Genomic DNA samples were isolated from fresh and green pomegranate leaves. Eight ISSR primers were used to determine genetic diversity between pomegranate populations. For the amplification of the cpDNA trnL‑F region, trne and trnf primers were used in PCR. In the ISSR-PCR analysis, all gel images were examined and the presence and absence of polymorphic bands were scored. In ISSR-PCR, a total of 55 bands were obtained. Polymorphism rate approximately 78% were determined. UPGMA dengogram consist of two clades. For cpDNA trnL‑F analysis, sequences were analysed using BioEdit and FinchTV programs. Both phylogenetic analyses and genetic distances were obtained using MEGA 6.0 program. Maximum likelihood and bootstrap trees were generated to determine genetic relationships between pomegranate populations. For populations, trnL‑F sequences were detected between 357 and 384 bases. In addition, trnL‑F sequences of some species of Lythraceae family from NCBI with pomegranate populations phylogenetic tree was constructed. Analysis of pomegranate populations based on ISSR-PCR and cpDNA trnL‑F sequences revealed that the rate of polymorphism obtained in the ISSR-PCR results were higher than the results obtained from the trnL‑F sequences.

在本研究中， 基于 ISSR-PCR 和叶绿体 DNA trn L-F 序列，对土耳其（爱琴海地区）Punica granatum L. 种群进行了分子遗传多样性和系统发育分析。从新鲜和绿色石榴叶中分离基因组 DNA 样本。八个 ISSR 引物用于确定石榴种群之间的遗传多样性。对于 cpDNA trn L-F 区域的扩增，trn e 和trnf 引物用于 PCR。在 ISSR-PCR 分析中，检查所有凝胶图像并对多态性条带的存在和不存在进行评分。在ISSR-PCR中，总共获得了55条带。多态性率大约为 78%。UPGMA denogram 由两个进化枝组成。对于 cpDNA trn L-F 分析，使用 BioEdit 和 FinchTV 程序分析序列。系统发育分析和遗传距离均使用 MEGA 6.0 程序获得。生成最大似然树和引导树以确定石榴种群之间的遗传关系。对于种群，在 357 到 384 个碱基之间检测到trn L-F 序列。此外，特恩利用石榴种群系统发育树构建了来自NCBI的某些睡莲科物种的L-F序列。基于 ISSR-PCR 和 cpDNA trn L-F 序列的石榴种群分析表明，ISSR-PCR 结果中获得的多态性率高于trn L-F 序列获得的结果。