Endophytic fungi are the endogenous micro-organisms to interacting with the plant cells, which do not exhibit any symptoms on the host plant and may produce some of the main secondary metabolites of the host plant cells. Ferula assa-foetida is a perennial and endemic medicinal plant of Iran, which is a rich source of sesquiterpene, coumarins, polysulfides and phenolic acids. In this study, 28 endophytic fungi isolates including Fusarium (60·7%), Aspergillus (7·1%), Alternaria (17·9%) and Plectosphaerella (7·1%) were isolated from F. assa-foetida root (57·1%), stem (32·1%) and leaf (10·8%) collected from Parvand protected area. Subsequently, their ability to produce phenolic acids was evaluated. The high amounts of total phenol (326·09 mg g⁻¹ of dry weight, DW), total flavonoid (901·11 mg g⁻¹ DW) and antioxidant activity (247·96 mg l⁻¹) were found in the supernatant fluid of SBUp1 isolate. The high-performance liquid chromatography analysis of 14 phenolic acids showed that rosmarinic acid (RA) is the main phenolic acid in the supernatant fluid of SBUp1 by 64·11 mg g⁻¹ DW confirmed by the liquid chromatography coupled with mass spectrometric analysis. According to morphological identification followed by phylogenetic study based on internal transcribed spacer (ITS) sequencing (ITS1-5.8S-ITS2) analysis, the SBUp1 isolate was identified as Alternaria tenuissima. Eventually, to our knowledge, it is the first document confirming A. tenuissima as an endophytic fungus of F. assa-foetida, which is a rich source of RA.

中文翻译：

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介绍 Alternaria tenuissima SBUp1，作为来自伊朗的 Ferula assa-foetida 的内生真菌，是迷迭香酸的丰富来源

内生真菌是与植物细胞相互作用的内源性微生物，在宿主植物上不表现出任何症状，并可能产生宿主植物细胞的一些主要次生代谢产物。Ferula assa-foetida是伊朗的一种多年生地方性药用植物，富含倍半萜、香豆素、多硫化物和酚酸。本研究从F. assa-foetida中分离出28 株内生真菌，包括镰刀菌(60·7%)、曲霉(7·1%)、链格孢(17·9%) 和Plectosphaerella (7·1%)从 Parvand 保护区收集的根 (57·1%)、茎 (32·1%) 和叶 (10·8%)。随后，评估了它们产生酚酸的能力。在上清液中发现了大量的总酚（326·09 mg g ^-1干重，DW）、总黄酮（901·11 mg g ^-1 DW）和抗氧化活性（247·96 mg l ^-1） SBUp1 分离液。14种酚酸的高效液相色谱分析表明，迷迭香酸(RA)是SBUp1上清液中的主要酚酸64·11 mg g ^-1DW 由液相色谱结合质谱分析证实。根据形态学鉴定和基于内部转录间隔（ITS）测序（ITS1-5.8S-ITS2）分析的系统发育研究，SBUp1分离株被鉴定为细链格孢。最终，据我们所知，这是第一个确认A. tenuissima是F. assa-foetida的内生真菌的文件，这是 RA 的丰富来源。