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Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
Biotechnology for Biofuels ( IF 6.3 ) Pub Date : 2021-07-23 , DOI: 10.1186/s13068-021-02011-y Tangwu Cui 1 , Bo Yuan 1 , Haiwei Guo 2 , Hua Tian 3 , Weimin Wang 1 , Yingqun Ma 1 , Changzhi Li 2 , Qiang Fei 1, 4
Biotechnology for Biofuels ( IF 6.3 ) Pub Date : 2021-07-23 , DOI: 10.1186/s13068-021-02011-y Tangwu Cui 1 , Bo Yuan 1 , Haiwei Guo 2 , Hua Tian 3 , Weimin Wang 1 , Yingqun Ma 1 , Changzhi Li 2 , Qiang Fei 1, 4
Affiliation
As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring with mild conditions and diversity, and has received great attention nowadays. In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. The maximum enzymatic activity of Lac and MnP was individually 18.06 U mL−1 and 13.58 U mL−1 along with a lignin degradation rate of 50% (wt/wt), which were achieved from batch cultivation of the consortium. The activities of Lac and MnP obtained from the consortium were both improved more than 40%, as compared with monocultures of L. betulina or T. versicolor under the same culture condition. The enhanced biodegradation performance was in accordance with the results observed from scanning electron microscope (SEM) of lignin samples before and after biodegradation, and secondary-ion mass spectrometry (SIMS). Finally, the analysis of heteronuclear single quantum coherence (HSQC) NMR and gas chromatography–mass spectrometry (GC–MS) provided a comprehensive product mapping of the lignin biodegradation, suggesting that the lignin has undergone depolymerization of the macromolecules, side-chain cleavage, and aromatic ring-opening reactions. Our results revealed a considerable escalation on the enzymatic activity obtained in a short period from the cultivation of the L. betulina or T. versicolor due to the enhanced microbial synergistic effects, providing a potential bioconversion route for lignin utilization.
中文翻译:
白腐真菌联合体增强木质素生物降解:微生物协同效应和产品图
作为木质纤维素生物质的主要成分之一,木质素被认为是世界上最丰富的可再生芳香族原料。与木质素降解的热或催化策略相比,生物转化是一种具有温和条件和多样性的有前途的方法,目前受到了极大的关注。在这项研究中,由白腐菌和花斑栓菌组成的白腐真菌联合体在微生物协同作用下提高漆酶 (Lac) 和锰过氧化物酶 (MnP) 的木质素分解酶活性。Lac 和 MnP 的最大酶活性分别为 18.06 U mL-1 和 13.58 U mL-1,木质素降解率为 50% (wt/wt),这是通过分批培养实现的。在相同培养条件下,与白桦茸或花斑紫杉的单一培养相比,从聚生体中获得的 Lac 和 MnP 的活性均提高了 40% 以上。增强的生物降解性能与生物降解前后木质素样品的扫描电子显微镜(SEM)和二次离子质谱(SIMS)观察到的结果一致。最后,异核单量子相干 (HSQC) NMR 和气相色谱-质谱 (GC-MS) 的分析提供了木质素生物降解的综合产物图谱,表明木质素经历了大分子的解聚、侧链裂解、和芳香开环反应。
更新日期:2021-07-23
中文翻译:
白腐真菌联合体增强木质素生物降解:微生物协同效应和产品图
作为木质纤维素生物质的主要成分之一,木质素被认为是世界上最丰富的可再生芳香族原料。与木质素降解的热或催化策略相比,生物转化是一种具有温和条件和多样性的有前途的方法,目前受到了极大的关注。在这项研究中,由白腐菌和花斑栓菌组成的白腐真菌联合体在微生物协同作用下提高漆酶 (Lac) 和锰过氧化物酶 (MnP) 的木质素分解酶活性。Lac 和 MnP 的最大酶活性分别为 18.06 U mL-1 和 13.58 U mL-1,木质素降解率为 50% (wt/wt),这是通过分批培养实现的。在相同培养条件下,与白桦茸或花斑紫杉的单一培养相比,从聚生体中获得的 Lac 和 MnP 的活性均提高了 40% 以上。增强的生物降解性能与生物降解前后木质素样品的扫描电子显微镜(SEM)和二次离子质谱(SIMS)观察到的结果一致。最后,异核单量子相干 (HSQC) NMR 和气相色谱-质谱 (GC-MS) 的分析提供了木质素生物降解的综合产物图谱,表明木质素经历了大分子的解聚、侧链裂解、和芳香开环反应。