Antagonistic activity of strains from Bacillus species has made them among the preferred agricultural biological control agents against phytopathogenic fungi. These microorganisms’ success is mostly based on the production of antagonistic secondary metabolites, mainly those of the non-ribosomal cyclic lipopeptides (CLPs) nature, which can affect phytopathogens directly (iturins and fengycins) or indirectly (surfactins and fengycins). However, abiotic factors in the target site can influence the behavior of the biocontrol traits, but to date, few studies attempting to decipher this kind of interaction have been conducted. This work aimed to evaluate the effect of temperature and culture medium on growth, antagonistic activity against Fusarium oxysporum f. sp. physali (Foph), and the profile of CLPs produced by Bacillus velezensis Bs006. The data showed that measured traits in Bs006 varied with temperature and medium interaction. The concentration of CLPs, as well as the antagonistic activity against Foph, was increased as the nutritional wealth, temperature, and time of incubation increased. The concentration of fengycins and iturins was higher than surfactins at high temperatures. However, a bacteriostatic effect was detected with a combination of Landy medium and 15 °C, which prevented both the biosynthesis of CLPs and the antagonistic activity. The results of this work highlight the importance of abiotic conditions of the target site where a biocontrol agent will be applied to stay active and develop its full antagonistic potential. This response by Bs006 could partly explain the variability of its biocontrol efficacy in the Foph-golden berry pathosystem.

芽孢杆菌属菌株的拮抗活性使其成为抗植物病原真菌的首选农业生物防治剂。这些微生物的成功主要基于拮抗次生代谢物的产生，主要是非核糖体环脂肽 (CLP) 性质的代谢产物，它们可以直接（iturins 和 fengycins）或间接（surfactins 和 fengycins）影响植物病原体。然而，目标部位的非生物因素会影响生物防治性状的行为，但迄今为止，很少有研究试图破译这种相互作用。这项工作旨在评估温度和培养基对生长的影响，以及对尖孢镰刀菌的拮抗活性。sp. 酸浆(Foph)，以及贝雷兹芽孢杆菌产生的 CLP 的概况BS006。数据表明，Bs006 中测量的性状随温度和介质相互作用而变化。CLP 的浓度以及对 Foph 的拮抗活性随着营养丰富、温度和孵育时间的增加而增加。fengycins和iturins的浓度在高温下高于surfactins。然而，在 Landy 培养基和 15°C 的组合下检测到抑菌效果，这阻止了 CLP 的生物合成和拮抗活性。这项工作的结果强调了目标部位的非生物条件的重要性，在这些条件下，生物防治剂将被应用以保持活性并发挥其全部拮抗潜力。Bs006 的这种反应可以部分解释其在 Foph-golden berry 病理系统中生物防治功效的可变性。