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A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters
Science Translational Medicine ( IF 17.1 ) Pub Date : 2021-08-11 , DOI: 10.1126/scitranslmed.abg1143
Yangtao Wu 1 , Xiaofen Huang 1 , Lunzhi Yuan 1 , Shaojuan Wang 1 , Yali Zhang 1 , Hualong Xiong 1 , Rirong Chen 2, 3 , Jian Ma 1 , Ruoyao Qi 1 , Meifeng Nie 1 , Jingjing Xu 1 , Zhigang Zhang 1 , Liqiang Chen 2, 3 , Min Wei 1 , Ming Zhou 1 , Minping Cai 2, 3 , Yang Shi 1 , Liang Zhang 1 , Huan Yu 2, 3 , Junping Hong 1 , Zikang Wang 1 , Yunda Hong 1 , Mingxi Yue 1 , Zonglin Li 1 , Dabing Chen 1 , Qingbing Zheng 1 , Shaowei Li 1 , Yixin Chen 1 , Tong Cheng 1 , Jun Zhang 1 , Tianying Zhang 1 , Huachen Zhu 2, 3 , Qinjian Zhao 1 , Quan Yuan 1 , Yi Guan 2, 3 , Ningshao Xia 1, 4
Affiliation  

Multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are necessary to respond to the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a protein subunit vaccine composed of spike ectodomain protein (StriFK) plus a nitrogen bisphosphonate–modified zinc-aluminum hybrid adjuvant (FH002C). StriFK-FH002C generated substantially higher neutralizing antibody titers in mice, hamsters, and cynomolgus monkeys than those observed in plasma isolated from COVID-19 convalescent individuals. StriFK-FH002C also induced both TH1- and TH2-polarized helper T cell responses in mice. In hamsters, StriFK-FH002C immunization protected animals against SARS-CoV-2 challenge, as shown by the absence of virus-induced weight loss, fewer symptoms of disease, and reduced lung pathology. Vaccination of hamsters with StriFK-FH002C also reduced within-cage virus transmission to unvaccinated, cohoused hamsters. In summary, StriFK-FH002C represents an effective, protein subunit–based SARS-CoV-2 vaccine candidate.



中文翻译:

一种重组刺突蛋白亚基疫苗赋予仓鼠抗 SARS-CoV-2 感染和传播的保护性免疫力

需要多种安全有效的疫苗来引发针对严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 的免疫反应,以应对持续的 2019 年冠状病毒病 (COVID-19) 大流行。在这里,我们开发了一种由刺突胞外域蛋白 (StriFK) 和氮双膦酸盐修饰的锌铝杂化佐剂 (FH002C) 组成的蛋白质亚单位疫苗。StriFK-FH002C 在小鼠、仓鼠和食蟹猴中产生的中和抗体滴度明显高于从 COVID-19 恢复期个体分离的血浆中观察到的抗体滴度。StriFK-FH002C 还诱导 T H 1- 和 T H小鼠中的 2 极化辅助性 T 细胞反应。在仓鼠中,StriFK-FH002C 免疫保护动物免受 SARS-CoV-2 的攻击,表现为没有病毒引起的体重减轻、疾病症状减少和肺部病变减轻。用 StriFK-FH002C 给仓鼠接种疫苗也减少了笼内病毒传播给未接种疫苗的合养仓鼠。总之,StriFK-FH002C 代表了一种有效的、基于蛋白质亚基的 SARS-CoV-2 候选疫苗。

更新日期:2021-08-12
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