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Morphology and phytochemistry of Teucrium chamaedrys L. (Lamiaceae) cultivated at the Ghirardi Botanic Garden (Lombardy, Northern Italy)
Flora ( IF 1.9 ) Pub Date : 2021-07-22 , DOI: 10.1016/j.flora.2021.151898
Claudia Giuliani 1, 2 , Martina Bottoni 1, 2 , Roberta Ascrizzi 3 , Fabrizia Milani 1, 2 , Alberto Spada 4 , Guido Flamini 3 , Gelsomina Fico 1, 2
Affiliation  

Teucrium chamaedrys L. (Lamiaceae) cultivated at the Ghirardi Botanic Garden (Lombardy, Northern Italy) was studied through a four-level research approach: 1) micromorphological and 2) histochemical to describe the features of the glandular trichomes on the vegetative and reproductive organs and the chemical nature of their secretory products, by means of Light, Fluorescence and Scanning Electron Microscopy; 3) phytochemical, with the first characterization of the volatiles spontaneously emitted by leaves and flowers of samples of Italian origin by means of Head-Space Solid Phase Micro-Extraction (HS-SPME) coupled with Gas Chromatography–Mass Spectrometry (GC-MS); 4) ecological, through the combination of the morphological and phytochemical results with literature data concerning the ecology of the major volatile compounds.

Three trichome morphotypes were observed, with a different distribution pattern on the vegetative and reproductive organs: peltates and short-stalked capitates, both ubiquitous on the whole plant, and long-stalked capitates, exclusive to the floral whorls. Both the peltates and the long-stalked capitates were recognized as the main sites of the terpene production. The HS-SPME analysis indicated that flowers displayed the most complex qualitative profile. Indeed, the vegetative and floral bouquets showed the predominance of sesquiterpene hydrocarbons and shared the main compounds, i.e., β-caryophyllene, γ-muurolene, and β-cubebene. From the literature data, a prevailing defensive action was highlighted both at the vegetative and reproductive organs level, along with the potential seductive role played by the floral bouquet.



中文翻译:

在 Ghirardi 植物园(意大利北部伦巴第)种植的 Teucrium chamaedrys L.(唇形科)的形态学和植物化学

茶树通过四个层次的研究方法研究了在 Ghirardi 植物园(意大利北部伦巴第)种植的唇形科(Lamiaceae):1)微形态学和 2)组织化学来描述营养和生殖器官上腺毛状体的特征以及通过光、荧光和扫描电子显微镜检查其分泌产物的化学性质;3) 植物化学,首次通过顶空固相微萃取 (HS-SPME) 结合气相色谱-质谱 (GC-MS) 对意大利样品的叶子和花朵自发释放的挥发物进行表征; 4) 生态学,通过将形态学和植物化学结果与有关主要挥发性化合物生态学的文献数据相结合。

观察到三种毛状体形态型,在营养和生殖器官上具有不同的分布模式:盾状和短柄头状体,在整个植株中都普遍存在,长柄头状体仅存在于花轮中。盾形头状体和长柄头状体均被认为是萜烯生产的主要场所。HS-SPME 分析表明花表现出最复杂的定性特征。事实上,植物和花卉花束显示出倍半萜烃的优势并共享主要化合物,即,β-石竹烯、γ-muurolene 和 β-cubebene。从文献数据,一个普遍的防守动作是在营养和生殖器官水平突出了,由花卉发挥的潜在作用诱人沿花束

更新日期:2021-07-29
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